Zeiss LSM700 - DZNE C1

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{{Equipment
 
{{Equipment
|system-number=MPI-CZ5
+
|system-number=DZNE-04
|system-name=Zeiss LSM 700 inverted
+
|system-name=Zeiss LSM 700 (DUO)
 
|type=Microscope
 
|type=Microscope
 
|category=Laser Scanning Confocal
 
|category=Laser Scanning Confocal
|facility=MPI-CBG LMF
+
|facility=DZNE - Imaging Platform
|building=MPI-CBG
+
|building=DZNE
|room=038a
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|room=2.08
 +
|Facility-logo=DZNE-Imaging-logo2-v6.png
 +
|Institute-logo=Logo-DZNE.gif
 
|description=Routine confocal workhorse for simple, standard and tile scan jobs.
 
|description=Routine confocal workhorse for simple, standard and tile scan jobs.
 
It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 +
 +
IMPORTANT: This System shares a common microscope stand and incubation chamber with the Spinning Disc (DUO)! Each of the two systems can be used independently of each other, but not simultaneously!
 
|applications=2D images, z-stacks, time lapse, bleaching and tile scans
 
|applications=2D images, z-stacks, time lapse, bleaching and tile scans
|image=Zeiss LSM 700.jpg
+
|image=DZNE_DUO-system.jpg
|stand-name=Zeiss - Axio Observer
+
|stand-name=Zeiss - Axio Examiner.Z1
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=-
 
|microscope=-
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|ill2=Laser DPSS 488 nm
 
|ill2=Laser DPSS 488 nm
 
|ill3=Laser DPSS 555nm
 
|ill3=Laser DPSS 555nm
|ill4=Laser DPSS 639nm
 
 
|ill5=Transmitted Light (Halogen)
 
|ill5=Transmitted Light (Halogen)
 
|ill6=Fluorescence (Metal Halide)
 
|ill6=Fluorescence (Metal Halide)
 
|detection=2 PMTs<br>
 
|detection=2 PMTs<br>
 
No transmission detector
 
No transmission detector
|reflectors=Dapi : EX 350/50 ; BS 395 ; EM LP 420 <br>
+
|reflectors=Dapi : EX G 365 ; BS 395 ; EM 445/50 <br>
FITC : EX 480/30 ; BS 505 ; EM 535/40 <br>
+
eGFP : EX 470/40 ; BS 495 ; EM 525/50 <br>
DsRed : EX 545/10 ; BS 555 ; EM 600/70 <br>
+
Cy3  : EX 545/25 ; BS 570 ; EM 605/70 <br>
GFP/RFP : EX  ; BS  ; EM
+
|features=<br>
|features=-
+
* number of different stage inserts to meet a variety of samples:
|software=ZEN 2010
+
- universal holder: for 1 slide or 1 dish <br>
|incubation=Not available
+
- multi slide holder: for up to 4 slides <br>
|link0=http://www.zeiss.de/C12567BE0045ACF1/Contents-Frame/A22821A1BDCAAE49C12574E80030638E
+
- multiwell holder
|inv=0   10.1.145.33
+
* fully integrated, dark incubation chamber
 +
<br>
 +
|software=ZEN 2011
 +
|incubation=Cage incubator (T+CO2)
 +
|inv=0
 
}}
 
}}

Revision as of 18:22, 5 December 2012

It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. IMPORTANT: This System shares a common microscope stand and incubation chamber with the Spinning Disc (DUO)! Each of the two systems can be used independently of each other, but not simultaneously!"Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. IMPORTANT: This System shares a common microscope stand and incubation chamber with the Spinning Disc (DUO)! Each of the two systems can be used independently of each other, but not simultaneously!" cannot be used as a page name in this wiki.

Suitable for 2D images, z-stacks, time lapse, bleaching and tile scans.
DZNE DUO-system.jpg


Directions

DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope Zeiss - Axio Examiner.Z1, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Neofluar 25x 0.8 Imm Corr
Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 555nm
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection 2 PMTs

No transmission detector

reflectors Dapi : EX G 365 ; BS 395 ; EM 445/50

eGFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3  : EX 545/25 ; BS 570 ; EM 605/70

features
  • number of different stage inserts to meet a variety of samples:

- universal holder: for 1 slide or 1 dish
- multi slide holder: for up to 4 slides
- multiwell holder

  • fully integrated, dark incubation chamber


"<br />

  • number of different stage inserts to meet a variety of samples:

- universal holder: for 1 slide or 1 dish <br /> - multi slide holder: for up to 4 slides <br /> - multiwell holder

  • fully integrated, dark incubation chamber

<br />" cannot be used as a page name in this wiki.

software ZEN 2011
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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