Zeiss LSM 700 feedback & bugs
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[[File:ZEN image format conversion colour bug.jpg|500px]] | [[File:ZEN image format conversion colour bug.jpg|500px]] | ||
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| + | == Comparison of file formats == | ||
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| + | * LSM5 - proprietory file format of the LSM 700; read by Fiji (file size in test 8MB) | ||
| + | * LSM4 - adapted TIFF format; colour problem as above; read by all graphics programmes which can read TIFF (file size in test 12MB) | ||
| + | * TIFF export - colour problem as above; all TIFF enabled graphics programmes (file size in test 12MB) | ||
| + | * JPEG - as noted above bigger than native LSM5 format w 9MB; colour bug | ||
Revision as of 16:48, 18 August 2011
The ZEN JPEG conversion is strange
Very strange. First, JPEGs are bigger than the original LSM files?? Converting via Fiji with very conservative settings of 90% quality gives me 1.7 MB JPEG from an 8.2 MB LSM file, while the ZEN JPEG is 9.4 MB. This makes no sense, since JPEG is a lossful compressor and most people use it to get more portable & versatile files of smaller size.
Also conversion does not stick to the colour scheme that is displayed/defined in ZEN. In my case blue in the LSM (left) gets converted to red in the JPEG (right). I also tried to save as TIFF - same colour problem here.
Comparison of file formats
- LSM5 - proprietory file format of the LSM 700; read by Fiji (file size in test 8MB)
- LSM4 - adapted TIFF format; colour problem as above; read by all graphics programmes which can read TIFF (file size in test 12MB)
- TIFF export - colour problem as above; all TIFF enabled graphics programmes (file size in test 12MB)
- JPEG - as noted above bigger than native LSM5 format w 9MB; colour bug
