Zeiss LSM 780 NLO - DZNE M1
From BioDIP
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{{Equipment | {{Equipment | ||
− | |system-number= | + | |system-number=DZNE-01 |
− | |system-name=Zeiss | + | |system-name=Zeiss LSM 780 NLO |
|type=Microscope | |type=Microscope | ||
|category=Multiphoton Laser Scanning | |category=Multiphoton Laser Scanning | ||
− | |facility= | + | |facility=DZNE - Imaging Platform |
− | |building= | + | |building=DZNE |
− | |room= | + | |room=208 |
|description=Zeiss LSM 780 NLO | |description=Zeiss LSM 780 NLO | ||
*Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. | *Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. | ||
− | *Single photon point scanning confocal system with | + | *Single photon point scanning confocal system with two conventional PMTs and two spectral GaAsP detector ("BiG") and transmitted light detector. Motorized stage for multi-point acquisition |
|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | ||
|image=MZ1-Zeiss 2photon upr.png | |image=MZ1-Zeiss 2photon upr.png | ||
− | |stand-name=Zeiss - Axio | + | |stand-name=Zeiss - Axio Examiner |
|stand=Upright | |stand=Upright | ||
|microscope=motorized XY stage, motorized Z-drive, | |microscope=motorized XY stage, motorized Z-drive, | ||
− | |obj1=Zeiss EC Plan | + | |obj1=Zeiss EC Plan Neofluar 2.5x 0.075 |
− | |obj2=Zeiss | + | |obj2=Zeiss Plan-Apochromat 10x 0.45 |
− | + | |obj3=Zeiss Plan-Apochromat 20x 0.8 | |
− | + | |obj4=Zeiss Plan-Apochromat 40x 0,95 korr. | |
− | | | + | |ill1=Fluorescence (Metal Halide, HXP, 120W) |
− | | | + | |
− | + | ||
− | + | ||
− | |ill1=Fluorescence (Metal Halide, | + | |
|ill2=Transmitted Light (Halogen) | |ill2=Transmitted Light (Halogen) | ||
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
− | |ill4=Laser Argon Multiline 458 | + | |ill4=Laser Argon Multiline 458, 488, 514 nm |
|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
− | |ill6 | + | |ill6=Laser HeNe 633 nm |
− | + | ||
|ill8=Laser Multiphoton Chameleon 700-1064 nm | |ill8=Laser Multiphoton Chameleon 700-1064 nm | ||
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | |detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> |
Revision as of 14:36, 12 March 2012
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3
Details
microscope | Zeiss - Axio Examiner, Upright stand, motorized XY stage, motorized Z-drive, | ||
objectives | |||
illumination | |||
detection | Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting 2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP | ||
reflectors | - | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
software | Zen 2010 B | ||
incubation | Not available | ||
links |
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inv.nr. | AIM System 2501000445 10.1.145.41 (IPv4) |