Zeiss LSM 780 NLO - DZNE M1
From BioDIP
(Difference between revisions)
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|description=Zeiss LSM 780 NLO | |description=Zeiss LSM 780 NLO | ||
*Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. | *Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. | ||
− | *Single photon point scanning confocal system with two conventional PMTs and | + | *Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition |
|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | ||
|image=MZ1-Zeiss 2photon upr.png | |image=MZ1-Zeiss 2photon upr.png | ||
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|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser HeNe 633 nm | |ill6=Laser HeNe 633 nm | ||
− | |ill8=Laser Multiphoton Chameleon | + | |ill8=Laser Multiphoton Chameleon VisionII 690-1064nm |
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | |detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | ||
'''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP | '''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP | ||
− | |reflectors= | + | |reflectors=Zeiss set 49: DAPI<br> |
+ | Zeiss set 38: GFP<br> | ||
+ | Zeiss set 43: Cy3 | ||
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
− | |software=Zen | + | |software=Zen "black" 2011 |
|incubation=Not available | |incubation=Not available | ||
− | + | |inv=AIM System 2504000174 | |
− | + | ||
− | |inv=AIM System | + | |
}} | }} |
Revision as of 14:46, 12 March 2012
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3
Details
microscope | Zeiss - Axio Examiner, Upright stand, motorized XY stage, motorized Z-drive, | ||
objectives | |||
illumination | |||
detection | Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting 2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP | ||
reflectors | Zeiss set 49: DAPI Zeiss set 38: GFP | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
software | Zen "black" 2011 | ||
incubation | Not available | ||
links |
| ||
inv.nr. | AIM System 2504000174 |