Zeiss Spinning Disc - DZNE SD1

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{{Equipment
 
{{Equipment
|system-number=DZNE-03
+
|system-number=DZNE-SD1
|system-name=SD3 - Zeiss Spinning Disc
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|system-name=Zeiss Spinning Disc
 
|type=Microscope
 
|type=Microscope
 
|category=Real-Time Confocal
 
|category=Real-Time Confocal
Line 7: Line 7:
 
|building=DZNE
 
|building=DZNE
 
|room=2.08
 
|room=2.08
|Institute-logo=Dzne-logo.png
+
|Facility-logo=DZNE-Imaging-logo2-v6.png
 +
|Institute-logo=Logo-DZNE.jpg
 
|description=Fast, "real-time" confocal imaging
 
|description=Fast, "real-time" confocal imaging
 +
 +
IMPORTANT: This system features an additional camera on the second camera port as well as a LED illumination system. It can therefore also be used as a conventional, fluorescence widefield microscope.
 
|applications=fast live confocal imaging
 
|applications=fast live confocal imaging
|image=MPI-SD3.jpg
+
|image=03 SpinningDisc DZNE.jpg
 
|stand-name=Zeiss - Axio Observer.Z1
 
|stand-name=Zeiss - Axio Observer.Z1
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=motorized, motorized xy scanning stage, motorized z-drive
 
|microscope=motorized, motorized xy scanning stage, motorized z-drive
|obj1=Zeiss Plan-Apochromat 5x/0.16
+
|obj1=Zeiss Plan-Apochromat 63x 1.4 Oil
 
|obj2=Zeiss Plan-Apochromat 10x 0.45
 
|obj2=Zeiss Plan-Apochromat 10x 0.45
 
|obj3=Zeiss Plan-Apochromat 20x 0.8
 
|obj3=Zeiss Plan-Apochromat 20x 0.8
 
|obj4=Zeiss Plan-Apochromat 40x 0,95 korr.
 
|obj4=Zeiss Plan-Apochromat 40x 0,95 korr.
 +
|obj5=Zeiss Plan-Apochromat 5x/0.16
 +
|obj6=Zeiss EC Plan Neofluar 2.5x 0.075
 
|ill1=Transmitted Light (LED)
 
|ill1=Transmitted Light (LED)
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
|ill3=Laser Diode 405 nm
+
|ill3=Colibri LED 365+470+590+625nm
|ill4=Laser Argon Multiline 458, 488, 514 nm
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|ill4=Laser Diode 405 nm
|ill5=Laser DPSS 561 nm
+
|ill5=Laser OPSL 488nm
|ill6=Laser DPSS 639nm
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|ill6=Laser DPSS 561 nm
|detection=Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole size: 24um, pinhole spacing: 240um
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|ill7=Laser Diode 635 nm
Zeiss AxiCam MRm with EM fitler wheel
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|detection=Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; physical pinhole radius: 25um, physical pinhole spacing: 250um
 
+
Zeiss Axiocam 506 mono: 2752x2208 Px, pixel size 4.54x4.54um, 19f/sec at full chip
Roper evolve 515 EMCCD with manual EM filter holder
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|reflectors=Dichroic Quattro RQFT 405+488+568+647 <br>
|reflectors=what filters?
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Emission filter wheel: <br> Pos1: BP 450/50<br> Pos2: BP 525/50 <br> Pos3: BP 629/62 <br> Pos4: DBP 527/54 + 645/60 <br> Pos5: BP 690/50
|features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing  = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm
+
|features=a number of different stage inserts is provided to meet a variety of samples:
|software=AxioVision from Zeiss
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- universal holder: for 1 slide or 1 dish <br>
|incubation=Cage incubator (T+CO2)
+
- multi slide holder: for up to 4 slides <br>
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD3_-_Zeiss_Spinning_Disc_-_startup_%26_shutdown
+
- multiwell holder
|link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedDYzUTM1djl2R2llc1d2ci0xYXRRNGc&output=html
+
<br>
|inv=MOZG04081820000  10.1.145.37
+
|software=ZEN Blue 2011 SP1
 +
|incubation=Stage incubator (T) + objective heater
 +
|inv=0
 
}}
 
}}

Latest revision as of 08:48, 21 June 2016

IMPORTANT: This system features an additional camera on the second camera port as well as a LED illumination system. It can therefore also be used as a conventional, fluorescence widefield microscope."Fast, "real-time" confocal imaging IMPORTANT: This system features an additional camera on the second camera port as well as a LED illumination system. It can therefore also be used as a conventional, fluorescence widefield microscope." cannot be used as a page name in this wiki.

Suitable for fast live confocal imaging.
03 SpinningDisc DZNE.jpg


[edit] Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, motorized, motorized xy scanning stage, motorized z-drive
objectives
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss Plan-Apochromat 5x/0.16
Zeiss EC Plan Neofluar 2.5x 0.075
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide, HXP, 120W)
Colibri LED 365+470+590+625nm
Laser Diode 405 nm
Laser OPSL 488nm
Laser DPSS 561 nm
Laser Diode 635 nm
detection Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; physical pinhole radius: 25um, physical pinhole spacing: 250um

Zeiss Axiocam 506 mono: 2752x2208 Px, pixel size 4.54x4.54um, 19f/sec at full chip

reflectors Dichroic Quattro RQFT 405+488+568+647

Emission filter wheel:
Pos1: BP 450/50
Pos2: BP 525/50
Pos3: BP 629/62
Pos4: DBP 527/54 + 645/60
Pos5: BP 690/50

features a number of different stage inserts is provided to meet a variety of samples:

- universal holder: for 1 slide or 1 dish
- multi slide holder: for up to 4 slides
- multiwell holder
"a number of different stage inserts is provided to meet a variety of samples: - universal holder: for 1 slide or 1 dish <br /> - multi slide holder: for up to 4 slides <br /> - multiwell holder <br />" cannot be used as a page name in this wiki.

software ZEN Blue 2011 SP1
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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