Zeiss Spinning Disc - DZNE SD1

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|ill2=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
 
|ill3=Laser Diode 405 nm
 
|ill3=Laser Diode 405 nm
|ill4=Laser Argon Multiline 458, 488, 514 nm
+
|ill4=Laser OPSL 488nm
 
|ill5=Laser DPSS 561 nm
 
|ill5=Laser DPSS 561 nm
|ill6=Laser DPSS 639nm
+
|ill6=Laser Diode 635 nm
|detection=Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole size: 24um, pinhole spacing: 240um
+
|detection=Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; pinhole size: 24um, pinhole spacing: 240um
Zeiss AxiCam MRm with EM fitler wheel
+
Zeiss AxiCam HRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip
  
 
Roper evolve 515 EMCCD with manual EM filter holder
 
Roper evolve 515 EMCCD with manual EM filter holder
|reflectors=what filters?
+
|reflectors=Emissionsfilterrad
|features=63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing  = 204.762 nm. Pinhole spacing is ??? nm and back projected pinhole size is ??? nm
+
Dichroic Quattro QDM 405+488+568+647
|software=AxioVision from Zeiss
+
Emission filter: DBP 527/54 + 645/60
|incubation=Cage incubator (T+CO2)
+
                BP 450/50
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-SD3_-_Zeiss_Spinning_Disc_-_startup_%26_shutdown
+
                BP 525/50
|link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedDYzUTM1djl2R2llc1d2ci0xYXRRNGc&output=html
+
                BP 629/62
|inv=MOZG04081820000  10.1.145.37
+
                BP 690/50
 +
|features=-
 +
|software=AxioVision Rel. 4.8.2
 +
|incubation=Not available
 +
|inv=0
 
}}
 
}}

Revision as of 16:56, 12 March 2012

Suitable for fast live confocal imaging.
MPI-SD3.jpg


Directions

[[image:|100px]] DZNE - Imaging Platform (DZNE, 2.08)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, motorized, motorized xy scanning stage, motorized z-drive
objectives
Zeiss Plan-Apochromat 5x/0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide, HXP, 120W)
Laser Diode 405 nm
Laser OPSL 488nm
Laser DPSS 561 nm
Laser Diode 635 nm
detection Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; pinhole size: 24um, pinhole spacing: 240um

Zeiss AxiCam HRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip

Roper evolve 515 EMCCD with manual EM filter holder

reflectors Emissionsfilterrad

Dichroic Quattro QDM 405+488+568+647 Emission filter: DBP 527/54 + 645/60

                BP 450/50
                BP 525/50
                BP 629/62
                BP 690/50
features -
software AxioVision Rel. 4.8.2
incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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