Zeiss Spinning Disc - DZNE SD1

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|ill1=Transmitted Light (LED)
 
|ill1=Transmitted Light (LED)
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Fluorescence (Metal Halide, HXP, 120W)
−
|ill3=Laser Diode 405 nm
+
|ill3=Fluorescence (LED)
−
|ill4=Laser OPSL 488nm
+
|ill4=Laser Diode 405 nm
−
|ill5=Laser DPSS 561 nm
+
|ill5=Laser OPSL 488nm
−
|ill6=Laser Diode 635 nm
+
|ill6=Laser DPSS 561 nm
 +
|ill7=Laser Diode 635 nm
 
|detection=Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; pinhole size: 24um, pinhole spacing: 240um
 
|detection=Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; pinhole size: 24um, pinhole spacing: 240um
 
Zeiss AxiCam HRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip
 
Zeiss AxiCam HRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip

Revision as of 15:37, 6 December 2012

IMPORTANT: This system features an additional camera on the second camera port as well as a LED illumination system. It can therefore also be used as a conventional, fluorescence widefield microscope!"Fast, "real-time" confocal imaging IMPORTANT: This system features an additional camera on the second camera port as well as a LED illumination system. It can therefore also be used as a conventional, fluorescence widefield microscope!" cannot be used as a page name in this wiki.

Suitable for fast live confocal imaging.
03 SpinningDisc DZNE.jpg


Directions

150px
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, motorized, motorized xy scanning stage, motorized z-drive
objectives
Zeiss Plan-Apochromat 5x/0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide, HXP, 120W)
Fluorescence (LED)
Laser Diode 405 nm
Laser OPSL 488nm
Laser DPSS 561 nm
Laser Diode 635 nm
detection Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; pinhole size: 24um, pinhole spacing: 240um

Zeiss AxiCam HRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip

reflectors Dichroic Quattro RQFT 405+488+568+647

Emission filter wheel:
Pos1: BP 450/50
Pos2: BP 525/50
Pos3: BP 629/62
Pos4: DBP 527/54 + 645/60
Pos5: BP 690/50

features a number of different stage inserts is provided to meet a variety of samples:

- universal holder: for 1 slide or 1 dish
- multi slide holder: for up to 4 slides
- multiwell holder
"a number of different stage inserts is provided to meet a variety of samples: - universal holder: for 1 slide or 1 dish <br /> - multi slide holder: for up to 4 slides <br /> - multiwell holder <br />" cannot be used as a page name in this wiki.

software ZEN Blue 2011
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0


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