Zeiss Spinning Disc Incubation- DZNE SD2

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{{Equipment
 
{{Equipment
|system-number=DZNE-04
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|system-number=DZNE-SD2
|system-name=Zeiss Spinning Disc (DUO)
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|system-name=Zeiss Spinning Disc Incubation
 
|type=Microscope
 
|type=Microscope
 
|category=Real-Time Confocal
 
|category=Real-Time Confocal
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|room=2.08
 
|room=2.08
 
|Facility-logo=DZNE-Imaging-logo2-v6.png
 
|Facility-logo=DZNE-Imaging-logo2-v6.png
|Institute-logo=Logo-DZNE.gif
+
|Institute-logo=Logo-DZNE.jpg
|description=IMPORTANT:
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|description=Fast, "real-time" confocal imaging
This system shares a common microscope stand and incubation chamber with the [[Zeiss DUO System LSM700 - DZNE 04|LSM 700]]! The two systems can be used independently of each other, but not simultaneously!
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|applications=fast "real time" live confocal imaging
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This Spinning disc system comes with a dark microscope stand incubation chamber, with which you can hold a stable temperature and CO2 environment.
 +
|applications=fast "real time" live confocal imaging,
 +
live cell imaging
 
|image=DZNE_DUO-system.jpg
 
|image=DZNE_DUO-system.jpg
 
|stand-name=Zeiss - Axio Observer.Z1
 
|stand-name=Zeiss - Axio Observer.Z1
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|ill5=Laser DPSS 561 nm
 
|ill5=Laser DPSS 561 nm
 
|ill6=Laser Diode 635 nm
 
|ill6=Laser Diode 635 nm
|detection=Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; pinhole size: 24um, pinhole spacing: 240um
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|detection=Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; physical pinhole radius: 50um, physical pinhole spacing: 250um
 
Zeiss AxiCam MRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip
 
Zeiss AxiCam MRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip
 
|reflectors=Dichroic Quattro RQFT 405+488+568+647 <br>
 
|reflectors=Dichroic Quattro RQFT 405+488+568+647 <br>
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* Advanced Experiment Designer
 
* Advanced Experiment Designer
 
<br>
 
<br>
|software=ZEN Blue 2011
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|software=ZEN Blue 2012
 
|incubation=Cage incubator (T+CO2)
 
|incubation=Cage incubator (T+CO2)
 
|inv=0
 
|inv=0
 
}}
 
}}

Latest revision as of 08:49, 21 June 2016

This Spinning disc system comes with a dark microscope stand incubation chamber, with which you can hold a stable temperature and CO2 environment."Fast, "real-time" confocal imaging This Spinning disc system comes with a dark microscope stand incubation chamber, with which you can hold a stable temperature and CO2 environment." cannot be used as a page name in this wiki.

Suitable for fast "real time" live confocal imaging, live cell imaging"fast "real time" live confocal imaging, live cell imaging" cannot be used as a page name in this wiki..
DZNE DUO-system.jpg


[edit] Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 2.08)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, motorized, motorized xy scanning stage, motorized z-drive
objectives
Zeiss EC Plan-Neofluar 5x 0.16 Ph1
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss Plan-Apochromat 20x 0.8
Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
illumination
Transmitted Light (LED)
Fluorescence (Metal Halide, HXP, 120W)
Laser Diode 405 nm
Laser OPSL 488nm
Laser DPSS 561 nm
Laser Diode 635 nm
detection Spinning disc scan head Yokogawa CSU-X1M 5000 dual cam; physical pinhole radius: 50um, physical pinhole spacing: 250um

Zeiss AxiCam MRm Rev.3: 1388x1040 Px, pixel size 6.45x6.45um, 12f/sec at full chip

reflectors Dichroic Quattro RQFT 405+488+568+647

Emission filter wheel:
Pos1: BP 450/50
Pos2: BP 525/50
Pos3: FE01 617/73
Pos5: BP 690/50

features
  • fast live cell imaging
  • number of different stage inserts to meet a variety of samples:

- universal holder: for 1 slide or 1 dish
- multi slide holder: for up to 4 slides
- multiwell holder

  • fully integrated, dark incubation chamber
  • Advanced Experiment Designer


"<br />

  • fast live cell imaging
  • number of different stage inserts to meet a variety of samples:

- universal holder: for 1 slide or 1 dish <br /> - multi slide holder: for up to 4 slides <br /> - multiwell holder

  • fully integrated, dark incubation chamber
  • Advanced Experiment Designer

<br />" cannot be used as a page name in this wiki.

software ZEN Blue 2012
incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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