SD1 - Andor Spinning Disc

Suitable for confocal imaging of EGFP, mCherry, dTomato, Draq5 and the like with high temporal and spatial resolution..

Directions

MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage
objectives	Olympus UPlanSApochromat 10x 0.4 Olympus UPlanFluar 20x 0.5 Olympus UPlanFluarN 60x 0.9 Olympus UPlanSApochromat 60x 1.20 W Olympus UPlanSApochromat 100x 1.4 Oil Olympus UPlanSApochromat 40x 0.9 Olympus UApochromat 40x 1.15 W Olympus UPlanFLN 40x 0.75 Ph2 Olympus UPlanFluar 60x 1.25 Oil Ph3 Olympus UApochromatN340 40x 1.15 W
illumination	Transmitted Light (Halogen) Fluorescence (HBO) Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm
detection	[[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): dichromatic mirrors: Quad band T-405/488/568/647 Triple band T-405/488/561 Single band T-488 physical pinhole radius: 25um, physical pinhole spacing: 250um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um Andor Neo sCMOS; dexel size of CMOS chip: 6.5um image pixel sizes (measured April 3rd 2012): for use with iXon: with 10x air objective: 1.695um/px (1x optovar); 1.055 um/px (1.6x opt.); with 40x air obj.: 0.418um/px (1x opt.), 0.26um/px (1.6x opt.); with 60x W obj.: 0.279um/px (1x opt.), 0.174um/px (1.6x opt.); with 100x oil obj.: 0.168um/px (1x opt.), 0.105um/px (1.6x opt.) for use with Neo: with 10x air objective: 0.692um/px (1x optovar); 0.432um/px (1.6x opt.); with 40x air obj.: 0.17um/px (1x opt.), 0.106um/px (1.6x opt.); with 60x W obj.: 0.114um/px (1x opt.), 0.071um/px (1.6x opt.); with 100x oil obj.: 0.068um/px (1x opt.), 0.043um/px (1.6x opt.) old measure pixel sizes before major upgrade 26th - 30th March 2012: image pixel size (measured again after major scope repair on March 7th 2011): with 100x objective: 0.175um (was 0.178um before 20110307); 100x obj. and 1.6x optovar: 0.109um (was 0.111um before 20110307); 60x obj.: 0.289um (was 0.288um before); 60x obj. and 1.6x opt.: 0.180um (was 0.181um before 20110307); 10x obj. : 1.747um, 10x obj. and 1.6x opt.: 1.089um camera projecting lens has about 0.92x (was 0.9x before 2011_03_07) magnification calculated from the measured pixel sizes]]
reflectors	Optical Insight DualView image splitter (currently not installed): holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Rotr filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer
features	micro point with 15Hz cutter laser with 365nm and 405nm dye cells; objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!"micro point with 15Hz cutter laser with 365nm and 405nm dye cells; objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!" cannot be used as a page name in this wiki.
software	[[software::iQ 2.9 + iQ 3.0 (both with Python) iQ3 user guide]]
incubation	Stage incubator (T) + objective heater
links	manual laser power values [- beam path] [ advanced system check]
inv.nr.	0 10.1.65.208

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