Zeiss LSM 780 NLO - DZNE M1

• Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
• Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
• Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
• Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.
  
  

Suitable for imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan.

Directions

DZNE - Imaging Platform (DZNE, 208)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

Details

microscope	Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive,
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss Plan-Apochromat 20x 0.8 Zeiss Plan-Apochromat 40x 0,95 korr. Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR Zeiss W Plan-Apochromat 20x/1.0
illumination	Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen) Laser Diode 405 nm Laser Argon Multiline 458, 488, 514 nm Laser DPSS 561 nm Laser HeNe 633 nm Laser Multiphoton Chameleon VisionII 690-1064nm
detection	Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting 2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm) Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)
reflectors	Zeiss set 49: DAPI Zeiss set 38: GFP Zeiss set 43: Cy3
features	simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software	Zen "black" 2011
incubation	Not available
links	manual [ laser power values] [ beam path] [ advanced system check]
inv.nr.	AIM System 2504000174

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