|
|
Line 10: |
Line 10: |
| |Institute-logo=Logo MPI-CBG.jpg | | |Institute-logo=Logo MPI-CBG.jpg |
| |description=Zeiss LSM 780 NLO | | |description=Zeiss LSM 780 NLO |
− | -Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
| + | *Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. |
− | -Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition
| + | *Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition |
| |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS |
| |image=MZ1 - Zeiss 2photon upright.jpg | | |image=MZ1 - Zeiss 2photon upright.jpg |
microscope
|
Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive,
|
objectives
|
- Zeiss EC Plan-Neofluar 10x 0.3
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss Clr Plan-Apochromat 10x 0.5 Scale
- Zeiss N-Achroplan 20x 0.5 W
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss W Plan-Apochromat 20x 1.0 DIC
- Zeiss Clr Plan-Neofluar 20x 1.0 Korr Clarity
- Zeiss LD SC Plan-Apochromat 20x 1.0 Korr Scale
- Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
- Zeiss C-Apochromat 40x 1.2 W
|
illumination
|
- Fluorescence (Metal Halide, HXP, 120W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 594 nm
- Laser HeNe 633 nm
- Laser Multiphoton Chameleon 700-1064 nm
-
-
|
detection
|
Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction
|
reflectors
|
Zeiss set 49: DAPI
Zeiss set 38 Endow: GFP
Zeiss set 45: HQ Texas Red
|
features
|
simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
|
software
|
Zen 2012 black
|
incubation
|
Not available
|
links
|
|
- [ beam path]
- [ advanced system check]
|
|
inv.nr.
|
AIM System 2501000445 10.1.145.41 (IPv4)
|