W3 - Zeiss CCD Upright

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{{Equipment
 
{{Equipment
| system-number       = 07
+
|system-number=MPI- W3
| system-name         = Zeiss CCD Upright
+
|system-name=Zeiss CCD Upright
| category             = Wide-Field
+
|type=Microscope
| stand                = upright
+
|category=Wide-Field
| image                = MPI07.jpg
+
|facility=MPI-CBG LMF
| facility             = MPI-CBG LMF
+
|building=MPI-CBG
| facility_image      = logo-lmf.jpg
+
|room=U35
| building            = MPI-CBG
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
| room                = -
+
|Institute-logo=Logo MPI-CBG.jpg
| description         = It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters.
+
|description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters.
| applications         = multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging
+
|applications=correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging, dark field microscopy
| microscope           = manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
+
|image=W3 - Zeiss CCD Upright.jpg
| objectives          = Plan Neofluar 5x/0.15 Ph1<br>Plan Neofluar 10/0.30<br>Achroplan 63x/0.80<br>Plan Neofluar 100x/1.30 Oil<br>
+
|stand-name=Zeiss - Axioplan 2
| illumination        = Halogen<br>HBO
+
|stand=Upright
| detection           = SPOT camera
+
|microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC; special dark field condenser
| reflectors           = DAPI (X BP 333-383, D LP 408, M BP 432-484)<br>FITC (X BP 446-494, DLP 513, M BP 516-567)<br>TRITC ( X BP 543-554, D LP 578, M BP 579-637<br>YFP 491-509, D LP 517, M BP 519-546)<br>CFP X BP 426-446, D LP 453,M BP 465-499)<br>Brightfield, Darkfield, Phase Contrast, and DIC<br>FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
+
|obj1=Zeiss Fluar 5x 0.25
| features             = -
+
|obj2=Zeiss Plan-Neofluar 10x 0.30
| incubation           = Bachoffer chamber bookable for this system when available  
+
|obj3=Zeiss Plan-Neofluar 40x 0.75
| link1               = -
+
|obj4=Zeiss Achroplan 63x 0.80
| link2               = -
+
|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil DIC
| inv                 = 401536
+
|obj6=Zeiss Plan-Apochromat 100x 1.4 Oil DIC
 +
|obj7=Zeiss Achroplan 20x 0.5 W Ph2
 +
|obj8=Zeiss C-Apochromat 63x 1.2 W
 +
|obj9=Zeiss EC Plan-Neofluar 100x 1.3 Oil Iris
 +
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
 +
|detection=Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2); imaging source fire wire monochrome camera DMK 31AF03 FC 8bit with 1024 x 768 pixels (4.65um^2)
 +
|reflectors=DAPI (X BP 333-383, D LP 408, M BP 432-484)<br>FITC (X BP 446-494, DLP 513, M BP 516-567)<br>TRITC ( X BP 543-554, D LP 578, M BP 579-637<br>YFP 491-509, D LP 517, M BP 519-546)<br>CFP X BP 426-446, D LP 453,M BP 465-499)<br>Brightfield, Darkfield, Phase Contrast, and DIC<br>FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
 +
|features=-
 +
|software=MetaVue, version 7.1.3
 +
|incubation=Not available
 +
|link1=-
 +
|link2=-
 +
|inv=401536
 +
|facility_image=logo-lmf.jpg
 +
|objectives=Plan Neofluar 5x/0.15 <br>Plan Neofluar 10/0.30<br>Plan Neofluar 25x/0.8 Ph2<br>Plan Neofluar 40x/0.75 Ph2<br>Achroplan 63x/0.80<br>Plan Neofluar 100x/1.30 Oil<br>
 +
|illumination=Halogen<br>HBO
 
}}
 
}}

Latest revision as of 15:35, 8 March 2016

Suitable for correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging, dark field microscopy.
W3 - Zeiss CCD Upright.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U35)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axioplan 2, Upright stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC; special dark field condenser
objectives
Zeiss Fluar 5x 0.25
Zeiss Plan-Neofluar 10x 0.30
Zeiss Plan-Neofluar 40x 0.75
Zeiss Achroplan 63x 0.80
Zeiss Plan-Apochromat 63x 1.4 Oil DIC
Zeiss Plan-Apochromat 100x 1.4 Oil DIC
Zeiss Achroplan 20x 0.5 W Ph2
Zeiss C-Apochromat 63x 1.2 W
Zeiss EC Plan-Neofluar 100x 1.3 Oil Iris
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
detection Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2); imaging source fire wire monochrome camera DMK 31AF03 FC 8bit with 1024 x 768 pixels (4.65um^2)
reflectors DAPI (X BP 333-383, D LP 408, M BP 432-484)
FITC (X BP 446-494, DLP 513, M BP 516-567)
TRITC ( X BP 543-554, D LP 578, M BP 579-637
YFP 491-509, D LP 517, M BP 519-546)
CFP X BP 426-446, D LP 453,M BP 465-499)
Brightfield, Darkfield, Phase Contrast, and DIC
FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
features -
software MetaVue, version 7.1.3
incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 401536



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