W3 - Zeiss CCD Upright
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=MPI- W3 |
|system-name=Zeiss CCD Upright | |system-name=Zeiss CCD Upright | ||
|type=Microscope | |type=Microscope | ||
| Line 6: | Line 6: | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
| − | |room= | + | |room=U35 |
| − | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| + | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters. | |description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters. | ||
| − | |applications=multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging | + | |applications=correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging, dark field microscopy |
| − | |image= | + | |image=W3 - Zeiss CCD Upright.jpg |
| − | |stand= | + | |stand-name=Zeiss - Axioplan 2 |
| − | |microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | + | |stand=Upright |
| − | | | + | |microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC; special dark field condenser |
| − | | | + | |obj1=Zeiss Fluar 5x 0.25 |
| − | |detection=Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2) | + | |obj2=Zeiss Plan-Neofluar 10x 0.30 |
| + | |obj3=Zeiss Plan-Neofluar 40x 0.75 | ||
| + | |obj4=Zeiss Achroplan 63x 0.80 | ||
| + | |obj5=Zeiss Plan-Apochromat 63x 1.4 Oil DIC | ||
| + | |obj6=Zeiss Plan-Apochromat 100x 1.4 Oil DIC | ||
| + | |obj7=Zeiss Achroplan 20x 0.5 W Ph2 | ||
| + | |obj8=Zeiss C-Apochromat 63x 1.2 W | ||
| + | |obj9=Zeiss EC Plan-Neofluar 100x 1.3 Oil Iris | ||
| + | |ill1=Transmitted Light (Halogen) | ||
| + | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
| + | |detection=Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2); imaging source fire wire monochrome camera DMK 31AF03 FC 8bit with 1024 x 768 pixels (4.65um^2) | ||
|reflectors=DAPI (X BP 333-383, D LP 408, M BP 432-484)<br>FITC (X BP 446-494, DLP 513, M BP 516-567)<br>TRITC ( X BP 543-554, D LP 578, M BP 579-637<br>YFP 491-509, D LP 517, M BP 519-546)<br>CFP X BP 426-446, D LP 453,M BP 465-499)<br>Brightfield, Darkfield, Phase Contrast, and DIC<br>FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621) | |reflectors=DAPI (X BP 333-383, D LP 408, M BP 432-484)<br>FITC (X BP 446-494, DLP 513, M BP 516-567)<br>TRITC ( X BP 543-554, D LP 578, M BP 579-637<br>YFP 491-509, D LP 517, M BP 519-546)<br>CFP X BP 426-446, D LP 453,M BP 465-499)<br>Brightfield, Darkfield, Phase Contrast, and DIC<br>FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621) | ||
|features=- | |features=- | ||
| − | |incubation= | + | |software=MetaVue, version 7.1.3 |
| + | |incubation=Not available | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
|inv=401536 | |inv=401536 | ||
| − | | | + | |facility_image=logo-lmf.jpg |
| − | | | + | |objectives=Plan Neofluar 5x/0.15 <br>Plan Neofluar 10/0.30<br>Plan Neofluar 25x/0.8 Ph2<br>Plan Neofluar 40x/0.75 Ph2<br>Achroplan 63x/0.80<br>Plan Neofluar 100x/1.30 Oil<br> |
| − | + | |illumination=Halogen<br>HBO | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | | | + | |
| − | + | ||
}} | }} | ||
Latest revision as of 15:35, 8 March 2016
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Zeiss - Axioplan 2, Upright stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC; special dark field condenser | ||
| objectives | |||
| illumination | |||
| detection | Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2); imaging source fire wire monochrome camera DMK 31AF03 FC 8bit with 1024 x 768 pixels (4.65um^2) | ||
| reflectors | DAPI (X BP 333-383, D LP 408, M BP 432-484) FITC (X BP 446-494, DLP 513, M BP 516-567) TRITC ( X BP 543-554, D LP 578, M BP 579-637 YFP 491-509, D LP 517, M BP 519-546) CFP X BP 426-446, D LP 453,M BP 465-499) Brightfield, Darkfield, Phase Contrast, and DIC FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621) | ||
| features | - | ||
| software | MetaVue, version 7.1.3 | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | 401536 |
