CO1 - Olympus Fluoview 1000

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| objectives          = 10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>60x/1.4 Oil PlanApo<br>60x/1.2 W UPlanApo
 
| objectives          = 10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>60x/1.4 Oil PlanApo<br>60x/1.2 W UPlanApo
 
| illumination        = Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br>
 
| illumination        = Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br>
| detection            = point scanner, 4 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral sliders and emission filters for detection range selection, pinhole for each detector
+
| detection            = point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole  
| reflectors          = Filter 1. U-MWU2[http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwu2.asp].  BP330-385  BA420  DM400   
+
| reflectors          = Filter 1. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwu2.asp U-MWU2].  BP330-385  BA420  DM400   
Filter 2.  [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490   BA520IF(High performance interference filter)   DM500<br>
+
Filter 2.  [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter)   DM500<br>
Filter 3. U-MWG2 [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp]. BP510-550  BA590  DM570
+
Filter 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550  BA590  DM570
| features            = simultaneous imaging using a 405nm picosecond  Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman [[averaging]], summarizing, scan zoom and rotation
+
| features            = Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation.Simultaneous imaging using a 405nm picosecond  Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman [[averaging]], summarizing, scan zoom and rotation
 
| incubation          = -
 
| incubation          = -
 
| link1                = [http://spreadsheets.google.com/pub?key=pFACvabR4mSmt7IA5ZOd9Yw LPM]
 
| link1                = [http://spreadsheets.google.com/pub?key=pFACvabR4mSmt7IA5ZOd9Yw LPM]
 
| link2                = -
 
| link2                = -
 
}}
 
}}

Revision as of 11:06, 28 August 2008

Suitable for ablation experiments.
MPI09.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
illumination
detection point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole
reflectors [[reflectors::Filter 1. U-MWU2. BP330-385 BA420 DM400

Filter 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500
Filter 3. U-MWG2. BP510-550 BA590 DM570]]

features [[features::Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation.Simultaneous imaging using a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation]]
software
incubation -
links
  • [ manual]
  • [LPM laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr.



[[Category:{{{type}}}]]






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