CO1 - Olympus Fluoview 1000
From BioDIP
(Difference between revisions)
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| objectives = 10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>60x/1.4 Oil PlanApo<br>60x/1.2 W UPlanApo | | objectives = 10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>60x/1.4 Oil PlanApo<br>60x/1.2 W UPlanApo | ||
| illumination = Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br> | | illumination = Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br> | ||
| − | | detection = point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole | + | | detection = point scanner, 4 confocal [http://en.wikipedia.org/wiki/Photomultiplier PMTs], two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole |
| reflectors = Filter 1. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwu2.asp U-MWU2]. BP330-385 BA420 DM400 | | reflectors = Filter 1. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwu2.asp U-MWU2]. BP330-385 BA420 DM400 | ||
Filter 2. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter) DM500<br> | Filter 2. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter) DM500<br> | ||
Filter 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550 BA590 DM570 | Filter 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550 BA590 DM570 | ||
| − | | features = Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation | + | | features = Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman [[averaging]], summarizing, scan zoom and rotation |
| incubation = - | | incubation = - | ||
| link1 = [http://spreadsheets.google.com/pub?key=pFACvabR4mSmt7IA5ZOd9Yw LPM] | | link1 = [http://spreadsheets.google.com/pub?key=pFACvabR4mSmt7IA5ZOd9Yw LPM] | ||
| link2 = - | | link2 = - | ||
}} | }} | ||
Revision as of 11:11, 28 August 2008
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives |
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| illumination |
| ||
| detection | [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]] | ||
| reflectors | [[reflectors::Filter 1. U-MWU2. BP330-385 BA420 DM400
Filter 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500 | ||
| features | [[features::Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation]] | ||
| software | |||
| incubation | - | ||
| links |
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| inv.nr. |
[[Category:{{{type}}}]]
