W3 - Zeiss CCD Upright

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Line 4: Line 4:
 
|type=Microscope
 
|type=Microscope
 
|category=Wide-Field
 
|category=Wide-Field
|facility=MPI-CBG LMF
+
|facility=MPI-CBG EM
 
|building=MPI-CBG
 
|building=MPI-CBG
 
|room=U48
 
|room=U48
 
|description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters.
 
|description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters.
|applications=multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging
+
|applications=correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging
 
|image=MPI07.jpg
 
|image=MPI07.jpg
 
|stand-name=Zeiss - Axioplan 2
 
|stand-name=Zeiss - Axioplan 2

Revision as of 11:23, 11 November 2013

Suitable for correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging.
MPI07.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG EM (MPI-CBG, U48)

Booking

https://java-srv1.mpi-cbg.de/cart/

Details

microscope Zeiss - Axioplan 2, Upright stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Zeiss Plan-Neofluar 100x 1.3 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
detection Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2)
reflectors DAPI (X BP 333-383, D LP 408, M BP 432-484)
FITC (X BP 446-494, DLP 513, M BP 516-567)
TRITC ( X BP 543-554, D LP 578, M BP 579-637
YFP 491-509, D LP 517, M BP 519-546)
CFP X BP 426-446, D LP 453,M BP 465-499)
Brightfield, Darkfield, Phase Contrast, and DIC
FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
features -
software -
incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 401536



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