|
|
Line 18: |
Line 18: |
| |microscope=motorized XY stage, motorized Z-drive, | | |microscope=motorized XY stage, motorized Z-drive, |
| |obj1=Zeiss EC Plan-Neofluar 10x 0.3 | | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 |
− | |obj2=Zeiss C-Apochromat 40x 1.2 W | + | |obj2=Zeiss Plan-Apochromat 10x 0.45 |
− | |obj3=Zeiss Plan-Apochromat 40x 1.0 W DIC | + | |obj3=Zeiss N-Achroplan 20x 0.5 W |
− | |obj4=Zeiss N-Achroplan 20x 0.5 W | + | |obj4=Zeiss Plan-Apochromat 20x 0.8 |
− | |obj5=Zeiss Plan-Apochromat 20x 0.8 | + | |obj5=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC |
− | |obj6=Zeiss Plan-Apochromat 10x 0.45 | + | |obj6=Zeiss C-Apochromat 40x 1.2 W |
− | |obj7=Zeiss Plan-Apochromat 63x 1.4 Oil DIC | + | |obj7=Zeiss Plan-Apochromat 40x 1.0 W DIC |
| |obj8=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC | | |obj8=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC |
− | |obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC | + | |obj9=Zeiss Plan-Apochromat 63x 1.4 Oil DIC |
| + | |
| |ill1=Fluorescence (Metal Halide, PRIOR, 200W) | | |ill1=Fluorescence (Metal Halide, PRIOR, 200W) |
| |ill2=Transmitted Light (Halogen) | | |ill2=Transmitted Light (Halogen) |
microscope
|
Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized Z-drive,
|
objectives
|
- Zeiss EC Plan-Neofluar 10x 0.3
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss N-Achroplan 20x 0.5 W
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
- Zeiss C-Apochromat 40x 1.2 W
- Zeiss Plan-Apochromat 40x 1.0 W DIC
- Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
- Zeiss Plan-Apochromat 63x 1.4 Oil DIC
-
|
illumination
|
- Fluorescence (Metal Halide, PRIOR, 200W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 477, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 594 nm
- Laser HeNe 633 nm
- Laser Multiphoton Chameleon 700-1064 nm
-
-
|
detection
|
Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP
|
reflectors
|
Zeiss set 49: DAPI
Zeiss set 38 Endow: GFP
Zeiss set 45: HQ Texas Red
|
features
|
simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
|
software
|
Zen 2012 black
|
incubation
|
Not available
|
links
|
|
- [ beam path]
- [ advanced system check]
|
|
inv.nr.
|
AIM System 2501000445 10.1.145.41 (IPv4)
|