SP5 MP, inverse, CRTD
From BioDIP
(Difference between revisions)
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|microscope=manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser | |microscope=manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser | ||
|obj1=Leica HC PL APO 10x 0.4 | |obj1=Leica HC PL APO 10x 0.4 | ||
− | |obj2=Leica HC PL APO 20x 0.7 | + | |obj2=Leica HC PL APO 20x 0.7 Imm Corr |
|obj3=Leica HCX PL APO 40x 1.25-0.75 Oil | |obj3=Leica HCX PL APO 40x 1.25-0.75 Oil | ||
|obj4=Leica HCX PL APO 63x 1.2 W | |obj4=Leica HCX PL APO 63x 1.2 W | ||
− | |obj5=- | + | |obj5=Leica HCX PL APO 63x 1.4-0.6 Oil |
|obj6=- | |obj6=- | ||
|obj7=- | |obj7=- | ||
|obj8=- | |obj8=- | ||
− | | | + | |ill1=Fluorescence (HBO) |
+ | |ill2=Transmitted Light (Halogen) | ||
+ | |ill3=Laser Diode 405 nm (pulsed) | ||
+ | |ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | ||
+ | |ill5=Laser DPSS 561 nm | ||
+ | |ill6=Laser HeNe 633 nm | ||
+ | |ill7=Laser Multiphoton 710-990 nm | ||
+ | |ill8=- | ||
|detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole<br>Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | |detection=point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole<br>Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | ||
− | |reflectors= | + | |reflectors=FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP<br>DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 |
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
|incubation=- | |incubation=- | ||
Line 30: | Line 37: | ||
|inv=0 | |inv=0 | ||
|objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | |objectives=10x/0.4 DIC<br>20x/0.7 DIC<br>40x/1.25-0.75 Oil DIC<br>63x/1.4-0.6 Oil DIC<br>63x/1.2 W Corr<br>63x/1.3 Glycerol 37°C, non Lambda-Blue (to be preferred fo MP imaging)<br>100x/1.4 Oil available on request | ||
+ | |illumination=HBO lamp<br>Halogen lamp<br>405nm diode laser<br>458, 476, 488, 496, 514nm argon laser<br>561nm DPSS laser<br>633nm HeNe<br>Multi-Photon : Mait Tai (710-990) | ||
}} | }} |
Revision as of 15:52, 7 September 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
microscope | , Inverted stand, manual XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC, Multi-Photon laser | ||
objectives | |||
illumination | |||
detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole Non Descan Detector for Multi Photon Imaging : FITC (525/50), TRITC (610/70) | ||
reflectors | FITC (I3) : EX 470/40 ; BS 510 ; EM LP 515 TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590 LP DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
software | |||
incubation | - | ||
links |
| ||
inv.nr. | 0 |