ASC Fluorescence and Brightfield Imaging
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File:Conva10xok-1.jpg with bar.jpg|Convallaria, Zeiss LSM 510, 10x/0.45 objective, 488 nm illumination, transmitted light detection | File:Conva10xok-1.jpg with bar.jpg|Convallaria, Zeiss LSM 510, 10x/0.45 objective, 488 nm illumination, transmitted light detection | ||
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Latest revision as of 09:50, 1 October 2009
First step of the system check. The purpose is to test the general performance of the system: Do the basic functions work fine? Does the acquisition software behave as it should? Can one set up Koehler illumination? Are the transmitted and the reflected beam-paths ok? Although this test does not represent high-resolution work, it might reveal general problems with the system.
[edit] requirements
- basic sample with fluorescence and contrast in transmitted light (i.e. Zeiss/Leica Convallaria demo sample)
[edit] acquisition
- set up the beam-path for fluorescence and transmitted light
- use basic air objective (i.e. 10x/0.4)
- acquire two images in basic resolution (i.e. 512x512)
[edit] analysis
- Does the illumination look reasonably homogeneous over the whole field of view in both images?
- Is the transmitted light image free of dirt particles?
- Are both images free of artifacts?
[edit] images
Convallaria, Zeiss LSM 510, 10x/0.45 objective, 488 nm excitation, BP505-550 detection, pinhole: 1 airy unit
Convallaria, Zeiss LSM 510, 10x/0.45 objective, 488 nm illumination, transmitted light detection
