ApoTome2, inverse, CRTD

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{{Equipment
 
{{Equipment
 
|system-number=BIO04
 
|system-number=BIO04
|system-name=ApoTome2, CRTD
+
|system-name=ApoTome2, inverse, CRTD
 
|type=Microscope
 
|type=Microscope
|category=Wide-Field
+
|category=Structured Illumination
|facility=Imaging@Biotec
+
|facility=Imaging@BIOTEC/CRTD
 
|building=CRTD
 
|building=CRTD
 
|room=-1.124
 
|room=-1.124
Line 11: Line 11:
 
|description=Our inverted ApoTome2 Microscope with hardware autofocus, colour and monochrome cameras is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome2 slider.
 
|description=Our inverted ApoTome2 Microscope with hardware autofocus, colour and monochrome cameras is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome2 slider.
 
|applications=multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus
 
|applications=multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus
|stand-name=Zeiss - Axio Observer
+
|image=ApoTome2, inverse, CRTD.jpg
 +
|stand-name=Zeiss - Axio Observer.Z1
 
|stand=Inverted
 
|stand=Inverted
|microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
+
|microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2), Mosaic (tile scans)
|obj1=Zeiss EC Plan-Neofluar 5x 0.15 Ph1
+
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
|obj3=Zeiss C-Apochromat 10x 0.45 W
+
|obj4=Zeiss LD Achroplan 20x 0.4 Ph2
|obj4=Zeiss C-Apochromat 40x 1.2 W
+
|obj5=Zeiss EC Plan-Neofluar 40x 0.6 Ph2
|obj5=Zeiss C-Apochromat 40x 1.2 W
+
|obj7=Zeiss C-Apochromat 63x 1.2 W
|ill1=Fluorescence (HBO)
+
|obj8=Zeiss EC Plan Neofluar 2.5x 0.075
|ill2=Fluorescence (Metal Halide, HXP, 120W)
+
|obj9=Zeiss Plan-Apochromat 20x 0.8
|ill3=Fluorescence (LED)
+
|ill1=Fluorescence (LED, 470nm)
|detection=Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color
+
|ill2=Fluorescence (LED, 590nm)
 +
|ill3=Fluorescence (Metal Halide, HXP, 120W)
 +
|ill4=Transmitted Light (Halogen)
 +
|detection=Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color <br>
 +
Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w <br>
 +
tube lens 1x and 1.6x
 
|reflectors=FS 49: EX BP 347/55; BS 395; EM  BP 445/50 (Dapi)<br>
 
|reflectors=FS 49: EX BP 347/55; BS 395; EM  BP 445/50 (Dapi)<br>
 
FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)<br>
 
FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)<br>
 
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
 
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
FS Cy5: (Cy5)<br>
+
FS Cy5: EX BP 662-738; BS 660; EM BP 700/75; (Cy5)<br>
FS F56-422: EX -; BS 500-555 & BP 615-675; EM BP 525/60 & BP 650/80; (GFP-DsRed)<br>
+
FS F56-422: EX BP 474/23 & BP 585/29; BS 495-555 & BP 610-690; EM BP 525/55 & BP 645/60; (GFP-DsRed)<br>
 
FS Analysator for DIC and Transmission
 
FS Analysator for DIC and Transmission
 
|features=-
 
|features=-
|software=-
+
|software=32 bit: AxioVision
 +
64 bit: Win7 ZEN blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=70836
 
|inv=70836
 
}}
 
}}

Latest revision as of 13:36, 17 October 2014

Suitable for multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus.
ApoTome2, inverse, CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.124)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2), Mosaic (tile scans)
objectives
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss EC Plan-Neofluar 40x 0.6 Ph2
Zeiss C-Apochromat 63x 1.2 W
Zeiss EC Plan Neofluar 2.5x 0.075
Zeiss Plan-Apochromat 20x 0.8
illumination
Fluorescence (LED, 470nm)
Fluorescence (LED, 590nm)
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color

Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w
tube lens 1x and 1.6x

reflectors FS 49: EX BP 347/55; BS 395; EM BP 445/50 (Dapi)

FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)
FS Cy5: EX BP 662-738; BS 660; EM BP 700/75; (Cy5)
FS F56-422: EX BP 474/23 & BP 585/29; BS 495-555 & BP 610-690; EM BP 525/55 & BP 645/60; (GFP-DsRed)
FS Analysator for DIC and Transmission

features -
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 70836



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