ApoTome2, inverse, CRTD

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|system-name=ApoTome2, inverse, CRTD
 
|system-name=ApoTome2, inverse, CRTD
 
|type=Microscope
 
|type=Microscope
|category=Wide-Field
+
|category=Structured Illumination
|facility=Imaging@Biotec
+
|facility=Imaging@BIOTEC/CRTD
 
|building=CRTD
 
|building=CRTD
 
|room=-1.124
 
|room=-1.124
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|applications=multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus
 
|applications=multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus
 
|image=ApoTome2, inverse, CRTD.jpg
 
|image=ApoTome2, inverse, CRTD.jpg
|stand-name=Zeiss - Axio Observer
+
|stand-name=Zeiss - Axio Observer.Z1
 
|stand=Inverted
 
|stand=Inverted
|microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
+
|microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2), Mosaic (tile scans)
|obj1=Zeiss EC Plan-Neofluar 5x 0.16 Ph1
+
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
 
|obj2=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
|obj3=Zeiss C-Apochromat 10x 0.45 W
+
|obj4=Zeiss LD Achroplan 20x 0.4 Ph2
|obj4=Zeiss Plan-Apochromat 20x 0.8 Ph2
+
|obj5=Zeiss EC Plan-Neofluar 40x 0.6 Ph2
|obj5=Zeiss C-Apochromat 40x 1.2 W
+
|obj7=Zeiss C-Apochromat 63x 1.2 W
|ill1=Fluorescence (LED)
+
|obj8=Zeiss EC Plan Neofluar 2.5x 0.075
|ill2=Fluorescence (Metal Halide, HXP, 120W)
+
|obj9=Zeiss Plan-Apochromat 20x 0.8
 +
|ill1=Fluorescence (LED, 470nm)
 +
|ill2=Fluorescence (LED, 590nm)
 +
|ill3=Fluorescence (Metal Halide, HXP, 120W)
 +
|ill4=Transmitted Light (Halogen)
 
|detection=Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color <br>
 
|detection=Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color <br>
Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w
+
Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w <br>
 
+
tube lens 1x and 1.6x
 
|reflectors=FS 49: EX BP 347/55; BS 395; EM  BP 445/50 (Dapi)<br>
 
|reflectors=FS 49: EX BP 347/55; BS 395; EM  BP 445/50 (Dapi)<br>
 
FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)<br>
 
FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)<br>
 
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
 
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
FS Cy5: (Cy5)<br>
+
FS Cy5: EX BP 662-738; BS 660; EM BP 700/75; (Cy5)<br>
FS F56-422: EX -; BS 500-555 & BP 615-675; EM BP 525/60 & BP 650/80; (GFP-DsRed)<br>
+
FS F56-422: EX BP 474/23 & BP 585/29; BS 495-555 & BP 610-690; EM BP 525/55 & BP 645/60; (GFP-DsRed)<br>
 
FS Analysator for DIC and Transmission
 
FS Analysator for DIC and Transmission
 
|features=-
 
|features=-
|software=-
+
|software=32 bit: AxioVision
 +
64 bit: Win7 ZEN blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=70836
 
|inv=70836
 
}}
 
}}

Latest revision as of 13:36, 17 October 2014

Suitable for multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus.
ApoTome2, inverse, CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.124)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2), Mosaic (tile scans)
objectives
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss EC Plan-Neofluar 40x 0.6 Ph2
Zeiss C-Apochromat 63x 1.2 W
Zeiss EC Plan Neofluar 2.5x 0.075
Zeiss Plan-Apochromat 20x 0.8
illumination
Fluorescence (LED, 470nm)
Fluorescence (LED, 590nm)
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color

Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w
tube lens 1x and 1.6x

reflectors FS 49: EX BP 347/55; BS 395; EM BP 445/50 (Dapi)

FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)
FS Cy5: EX BP 662-738; BS 660; EM BP 700/75; (Cy5)
FS F56-422: EX BP 474/23 & BP 585/29; BS 495-555 & BP 610-690; EM BP 525/55 & BP 645/60; (GFP-DsRed)
FS Analysator for DIC and Transmission

features -
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 70836



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