BasicsCoursePhDprog-PeterEvennetMay2011

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| 11:20||12:30||align="center"|SESSION 1 <br>Theory|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses (Lens demo fun)<br>Lens aberrations (see colour fringes of lighting image with simple lens)<br>Magnification<br> Numerical aperture <br>Microscope illumination<br>Conjugate planes||align="center" |PETER EVENNETT <br>LMF TEAM
 
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Revision as of 10:41, 12 April 2011

Contents

PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

Setup -Fri 13 May

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (16th May)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 09:30 FINAL PREP Test everything works LMF TEAM
9:30 11:05 INTRODUCTION COURSE INTRO
WHO is WHO? Round table		 LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 12:30 SESSION 1
Theory		 PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses (Lens demo fun)
Lens aberrations (see colour fringes of lighting image with simple lens)
Magnification
Numerical aperture
Microscope illumination
Conjugate planes		 PETER EVENNETT
LMF TEAM
12:30 13:30 LUNCH LUNCH LUNCH
13:30 14:15 SESSION 1
Practice
Rotation		 Milky Glass Block demo
conjugate planes on bench microscope
NA measurements		 LMF TEAM
14:15 15:30 SESSION 2
Theory		 Koehler Illumination
(Microscope alignment)		 PETER EVENNETT
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 2
Practice		 Koehler illumination
(Microscope alignment)		 PETER EVENNETT
LMF TEAM

DAY TWO (May 17th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap Recap day 1 LMF TEAM
9:30 10:45 SESSION 1
Theory & Practice		 Resolution
Diffraction 		PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:00 SESSION 2
Theory		 Bright Field
Dark Field		 PETER EVENNETT
LMF TEAM
15:00 16:00 SESSION 2
Practice		 Bright Field
Dark Field		 PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
16:15 17:00 SESSION 2
Theory		 Phase Contrast microscopy PETER EVENNETT
LMF TEAM
17:00 18:00 SESSION 2
Practice		 Phase Contrast microscopy PETER EVENNETT
LMF TEAM

DAY THREE (May 18th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 2 LMF TEAM
9:30 11:30 SESSION 1
Theory & Practice		 Objective reading
Objective cleaning		 PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice		 Polarized light microscopy
Differential Interference Contrast (DIC)		 PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice		 Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy 		PETER EVENNETT
LMF TEAM

DAY FOUR (May 19th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:00 9:30 Recap day 3 LMF TEAM
9:30 10:20 SESSION 1
Theory		 Question session
Fundamentals concepts of fluorescence 		LMF TEAM
Dan		 - -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice		 Fluorescence Microscopy LMF TEAM
Dan? Sike? Jan?		 • Mercury/Xenon/MetalHalide lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)		 • Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice		 Imaging with CCD and Quantitative Imaging LMF TEAM
Britta/Dan		 • CCD
• Cameras 		• CCD chip
• RGB Slider Spot Camera setup
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice		 Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta / Silke (*)		 • Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast 		• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM
• Fluorescence Sample slides (Kidney / Cells

DAY FIVE (May 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 4 LMF TEAM
9:30 10:20 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Small Groups Discussion		 What techniques might be good for me
(Own Projects) 		LMF TEAM
Dan/Jan/Silke/Britta/Pete?
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

OFFICE DUTIES

- LMF Members available for users (16th May to 20th May) -

FIX ME!!!

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Silke Jan Jan Britta
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Dan Silke -
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