BasicsCoursePhDprog-PeterEvennetMay2011

Latest revision as of 18:45, 29 April 2011

[edit] PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

[edit] Setup -Fri 13 May

Setup - all lmf staff move stuff to galleria Equipment needed

Microscopes
Optical bench
Spinning disk gadget
Polarazing sheets
Diffraction Grids
Fluorescent samples
Fluorescent Beads (Prepare slides)
First day Handout for students
Light torches

[edit] DAY ONE (16th May)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	09:30	FINAL PREP	Test everything works	LMF TEAM
9:30	10:30	INTRODUCTION	COURSE INTRO WHO is WHO? Round table	LMF TEAM
10:30	11:00	Session 1	Historical aspects of light microscopy	Peter Evennett
11:00	11:15	BREAK	BREAK	BREAK
11:15	12:30	SESSION 1 Theory	PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses (Lens demo fun) Lens aberrations (see colour fringes of lighting image with simple lens) Magnification Numerical aperture Microscope illumination Conjugate planes	PETER EVENNETT LMF TEAM
12:30	13:30	LUNCH	LUNCH	LUNCH
13:30	14:15	SESSION 1 Practice Rotation	Milky Glass Block demo Conjugate planes on bench microscope NA measurements	Dan Jan Silke
14:15	15:30	SESSION 2 Theory	Koehler Illumination (Microscope alignment)	PETER EVENNETT
15:30	15:45	BREAK	BREAK	BREAK
15:45	18:00	SESSION 2 Practice	Koehler illumination (Microscope alignment)	PETER EVENNETT LMF TEAM

[edit] DAY TWO (May 17th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	9:45	Recap	day 1	LMF TEAM
9:45	11:00	SESSION 1 Theory & Practice	Resolution Diffraction	PETER EVENNETT LMF TEAM
11:00	11:15	BREAK	COFFEE BREAK
11:15	13:15	DEMONSTRATION	Abbe´s diffraction experiments	PETER EVENNETT LMF TEAM
13:15	14:15	BREAK	LUNCH
14:15	15:15	SESSION 2 Theory	Bright Field Dark Field	PETER EVENNETT LMF TEAM
15:15	16:15	SESSION 2 Practice	Bright Field Dark Field	PETER EVENNETT LMF TEAM
16:15	16:30	BREAK	COFFEE BREAK
16:30	17:15	SESSION 2 Theory	Phase Contrast microscopy	PETER EVENNETT LMF TEAM
17:15	18:15	SESSION 2 Practice	Phase Contrast microscopy	PETER EVENNETT LMF TEAM

[edit] DAY THREE (May 18th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	9:45	Recap	day 2	LMF TEAM
9:45	10:45	SESSION 1 Theory	Objective reading Objective cleaning	PETER EVENNETT LMF TEAM
10:45	11:00	BREAK	BREAK	BREAK
11:00	12:00	SESSION 1 Practice Group rotation	Cover glasses and sample mounting Objective reading Objective cleaning	Dan Britta, Silke Jan
12:00	13:15	SESSION 2 Theory & Practice	Polarized light microscopy	PETER EVENNETT LMF TEAM
13:15	14:15	BREAK	LUNCH
14:15	15:30	SESSION 3 Theory & Practice	Differential Interference Contrast (DIC)	PETER EVENNETT LMF TEAM
15:30	15:45	BREAK	BREAK	BREAK
15:45	16:15	Question round	Questions to Peter Evennett	PETER EVENNETT LMF TEAM
16:15	18:15	SESSION 4 Theory & Demo	What is a pixel Analog to Digital conversion Nuyquist-Shannon	Dan

[edit] DAY FOUR (May 19th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)	DEMO Activities	DEMO materials
9:00	9:45	Recap	day 3	LMF TEAM
9:45	10:30	SESSION 1 Theory	Fundamental concepts of fluorescence	LMF TEAM Dan	-	-
10:30	10:45	BREAK	BREAK	BREAK	BREAK	BREAK
10:45	12:15	SESSION 2 Theory & Practice	Fluorescence Microscopy	LMF TEAM Silke	• Light sources • Lamp houses • Spectra • Filters • Filter cubes • Filter spectral charts of each microscope (laminated)	• Spare lamp houses • Lamps • Ocean optics equipment • Four (4) different Filter-sets (cubes), with the appropriate descriptions • diagramms for drawing filter/fluorophore spectra
12:15	13:15	SESSION 2 Practice	Fluorescence imaging at microscopes	LMF TEAM	-	• Fluorescent labeled samples
13:15	14:15	BREAK	LUNCH	LUNCH	LUNCH	LUNCH
14:15	15:45	SESSION 3 Theory & Practice	Imaging with CCD and Quantitative Imaging	LMF TEAM Britta/Dan	• CCD • Cameras	• CCD chip • RGB Slider Spot Camera setup
15:45	16:00	BREAK	BREAK
16:00	18:00	SESSION 4 Practice	Imaging with CCD cameras using LMF systems	LMF TEAM	• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast	• Microscopes: DVcore(Dan, 3 students) pDV (Silke, 2 students) Inverted CCD (Pete, 2 students) N-Storm (Britta, 2 students) self-built-syst (Jan, 3 students)) • Fluorescence Sample slides (Kidney / Cells)
19:00	open end	Dinner	with Peter	LMF TEAM

[edit] DAY FIVE (May 20th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	9:45	Recap	day 4	LMF TEAM
9:45	10:45	SESSION 1 Theory & Demo	Optical sectioning methods Pros&Cons	LMF TEAM Dan / Jan
10:45	11:00	BREAK	BREAK	BREAK
11:00	13:15	SESSION 1 Theory & Demo	Optical sectioning methods Pros&Cons	LMF TEAM Dan / Jan
13:15	14:15	BREAK	LUNCH
14:15	15:00	SESSION 2 Small Groups Discussion	What techniques might be good for me (Own Projects)	LMF TEAM Dan/Jan/Silke/Britta/Pete?
15:00	15:15	BREAK	BREAK	BREAK
15:15	16:00	SESSION 3	Course evaluation	LMF TEAM

[edit] OFFICE DUTIES

[edit] - LMF Members available for users (16th May to 20th May) -

No office duty this time due to lack of staff. One member of the LMF will always carry the helpline phone, though.

FROM	TO	MONDAY	TUESDAY	WEDNESDAY	THURSDAY	FRIDAY

9:30	12:30	-	---	---	---	---
12:30	13:30	L	U	N	C	H

13:30	18:00	---	---	---	---	-

BasicsCoursePhDprog-PeterEvennetMay2011

Latest revision as of 18:45, 29 April 2011

Contents

[edit] PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

[edit] Setup -Fri 13 May

[edit] DAY ONE (16th May)

[edit] DAY TWO (May 17th)

[edit] DAY THREE (May 18th)

[edit] DAY FOUR (May 19th)

[edit] DAY FIVE (May 20th)

[edit] OFFICE DUTIES

[edit] - LMF Members available for users (16th May to 20th May) -

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@@ Line 29: / Line 29: @@
 |-
 | 9:00||09:30||align="center"|FINAL PREP||align="center" | Test everything works ||align="center" |LMF TEAM
-|-| ||||||||
 |-
-| 9:30||11:05||align="center"|INTRODUCTION||align="center" | COURSE INTRO <br> WHO is WHO? Round table||align="center" |LMF TEAM
 |-
-| ||||||||
+| 9:30||10:30||align="center"|INTRODUCTION||align="center" | COURSE INTRO <br> WHO is WHO? Round table||align="center" |LMF TEAM
 |-
-| style="background:DarkKhaki;"|'''11:05'''
+|-
-| style="background:DarkKhaki;"|'''11:20'''
+| 10:30||11:00||align="center"|Session 1||align="center" | Historical aspects of light microscopy||align="center" |Peter Evennett
+|-
+|-
+| style="background:DarkKhaki;"|'''11:00'''
+| style="background:DarkKhaki;"|'''11:15'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
-| ||||||||
-|-
-| 11:20||12:30||align="center"|SESSION 1 <br>Theory|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses (Lens demo fun)<br>Lens aberrations (see colour fringes of lighting image with simple lens)<br>Magnification<br> Numerical aperture <br>Microscope illumination<br>Conjugate planes<br>Koehler illumination (Microscope Alignment Practice 1)||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
+| 11:15||12:30||align="center"|SESSION 1 <br>Theory|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY<br> Historical aspects of microscopy<br>Introduction to lenses (Lens demo fun)<br>Lens aberrations (see colour fringes of lighting image with simple lens)<br>Magnification<br> Numerical aperture <br>Microscope illumination<br>Conjugate planes||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
 |-
@@ Line 56: / Line 52: @@
 |align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 |-
-| ||||||||
 |-
-| 13:30||14:15||align="center"|SESSION 1<br>Practice<br> Rotation|| align="center" |Milky Glass Block demo <br> conjugate planes on bench microscope<br>NA measurements||align="center" |PETER EVENNETT <br>LMF TEAM
+| 13:30||14:15||align="center"|SESSION 1<br>Practice<br> Rotation|| align="center" |Milky Glass Block demo <br> Conjugate planes on bench microscope<br>NA measurements||align="center" |Dan<br>Jan<br>Silke
 |-
-| ||||||||
 |-
-|14:15||15:30||align="center"|SESSION 2<br>Theory||align="center" |Koehler Illumination <br> Microscope alignment||align="center" |PETER EVENNETT <br>LMF TEAM
+|14:15||15:30||align="center"|SESSION 2<br>Theory||align="center" |Koehler Illumination <br> (Microscope alignment)||align="center" |PETER EVENNETT
 |-
-| ||||||||
 |-
 | style="background:DarkKhaki;"|'''15:30'''
@@ Line 71: / Line 64: @@
 |align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
-| ||||||||
 |-
-| 15:45||18:00||align="center"|SESSION 3<br>Theory & Practice|| align="center" |PRINCIPLES OF LIGHT MICROSCOPY||align="center" |PETER EVENNETT <br>LMF TEAM
+| 15:45||18:00||align="center"|SESSION 2<br>Practice|| align="center" |Koehler illumination<br>(Microscope alignment)||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
 |}
@@ Line 88: / Line 79: @@
 | align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 |-
-| 9:00||9:30||align="center"|Recap ||align="center" |  day 1  ||align="center" |LMF TEAM
+| 9:00||9:45||align="center"|Recap ||align="center" |  day 1  ||align="center" |LMF TEAM
 |-
-| 9:30||10:45||align="center"|SESSION 1 <br>Theory & Practice||align="center" |  Resolution<br>Diffraction  ||align="center" |PETER EVENNETT <br>LMF TEAM
+| 9:45||11:00||align="center"|SESSION 1 <br>Theory & Practice||align="center" |  Resolution<br>Diffraction  ||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
 |-
 |--
 |-
-| style="background:DarkKhaki;"|'''10:45'''
 | style="background:DarkKhaki;"|'''11:00'''
+| style="background:DarkKhaki;"|'''11:15'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''     '''
 |-
-| 11:00||13:00||align="center"|DEMONSTRATION || align="center"|Abbe´s diffraction experiments<br>||align="center"|PETER EVENNETT <br>LMF TEAM
+| 11:15||13:15||align="center"|DEMONSTRATION || align="center"|Abbe´s diffraction experiments<br>||align="center"|PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
 |-
-| style="background:DarkKhaki;"|'''13:00'''
+| style="background:DarkKhaki;"|'''13:15'''
-| style="background:DarkKhaki;"|'''14:00'''
+| style="background:DarkKhaki;"|'''14:15'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''     '''
 |-
-| ||||||||
 |-
-| 14:00||16:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+| 14:15||15:15||align="center"|SESSION 2<br>Theory|| align="center"|Bright Field<br> Dark Field||align="center" |PETER EVENNETT <br>LMF TEAM
+|-
+|-
+| 15:15||16:15||align="center"|SESSION 2<br>Practice|| align="center"|Bright Field<br> Dark Field||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
 |-
-| style="background:DarkKhaki;"|'''16:00'''
 | style="background:DarkKhaki;"|'''16:15'''
+| style="background:DarkKhaki;"|'''16:30'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''     '''
 |-
-| 16:15||18:00||align="center"|SESSION 2<br>Theory & Practice|| align="center"|Bright Field<br> Dark Field<br> Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
+| 16:30||17:15||align="center"|SESSION 2<br>Theory|| align="center"|Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+|-
+|-
+| 17:15||18:15||align="center"|SESSION 2<br>Practice|| align="center"|Phase Contrast microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
 |}
@@ Line 135: / Line 128: @@
 | align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 |-
-| 9:00||9:30||align="center"|Recap ||align="center" |  day 2  ||align="center" |LMF TEAM
+| 9:00||9:45||align="center"|Recap ||align="center" |  day 2  ||align="center" |LMF TEAM
 |-
-| 9:30||11:30||align="center"|SESSION 1<br>Theory & Practice||align="center" | Objective reading<br> Objective cleaning||align="center" |PETER EVENNETT <br>LMF TEAM
+| 9:45||10:45||align="center"|SESSION 1<br>Theory||align="center" | Objective reading<br> Objective cleaning||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
 |-
-| style="background:DarkKhaki;"|'''11:30'''
+| style="background:DarkKhaki;"|'''10:45'''
-| style="background:DarkKhaki;"|'''11:45'''
+| style="background:DarkKhaki;"|'''11:00'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
-| ||||||||
 |-
-| 11:45||13:00||align="center"|SESSION 2<br>Theory & Practice||align="center" | Polarized light microscopy<br>Differential Interference Contrast (DIC)||align="center" |PETER EVENNETT <br>LMF TEAM
+| 11:00||12:00||align="center"|SESSION 1<br>Practice<br>Group rotation||align="center" | Cover glasses and sample mounting<br>Objective reading<br> Objective cleaning||align="center" |Dan<br>Britta, Silke<br>Jan
 |-
-| ||||||||
 |-
+| 12:00||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Polarized light microscopy||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| style="background:DarkKhaki;"|'''13:00'''
+|-
-| style="background:DarkKhaki;"|'''14:00'''
+| style="background:DarkKhaki;"|'''13:15'''
+| style="background:DarkKhaki;"|'''14:15'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''     '''
 |-
-| ||||||||
 |-
-| 14:00||15:15||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br>Objective cleaning<br>Digital photomicrography<br>Introduction to Confocal Microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+| 14:15||15:30||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| ||||||||
 |-
-| style="background:DarkKhaki;"|'''15:15'''
 | style="background:DarkKhaki;"|'''15:30'''
+| style="background:DarkKhaki;"|'''15:45'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
-| ||||||||
+| 15:45||16:15||align="center"|Question round|| align="center"|Questions to Peter Evennett||align="center" |PETER EVENNETT <br>LMF TEAM
 |-
-| 15:30||18:00||align="center"|SESSION 4<br>Theory & Practice|| align="center"|Differential Interference Contrast (DIC)<br>Objective cleaning<br>Digital photomicrography<br>Introduction to Confocal Microscopy ||align="center" |PETER EVENNETT <br>LMF TEAM
+| 16:15||18:15||align="center"|SESSION 4<br>Theory & Demo|| align="center"|What is a pixel<br>Analog to Digital conversion<br>Nuyquist-Shannon||align="center" |Dan
 |-
-| ||||||||
 |}
@@ Line 188: / Line 177: @@
 | align="center" style="background:#f0f0f0;"|'''DEMO materials'''
 |-
-| 9:00||9:30||align="center"|Recap ||align="center" |  day 3  ||align="center" |LMF TEAM ||||
+| 9:00||9:45||align="center"|Recap ||align="center" |  day 3  ||align="center" |LMF TEAM ||||
 |-
-| 9:30||10:20||align="center"|SESSION 1<br>Theory||align="center" | Question session <br> Fundamentals concepts of fluorescence ||align="center" |LMF TEAM<br>Dan||align="center" |- ||align="center" |-
+| 9:45||10:30||align="center"|SESSION 1<br>Theory||align="center" | Fundamental concepts of fluorescence ||align="center" |LMF TEAM<br>Dan||align="center" |- ||align="center" |-
 |-
-| ||||||||||||
 |-
-| style="background:DarkKhaki;"|'''10:20'''
+| style="background:DarkKhaki;"|'''10:30'''
-| style="background:DarkKhaki;"|'''10:55'''
+| style="background:DarkKhaki;"|'''10:45'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
-| ||||||||||||
 |-
-| 10:55||13:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Dan? Sike? Jan?||align="center" |•	Mercury/Xenon/MetalHalide lamp<br>•	Lamp houses <br>• Spectra <br>•	Filters <br> • Filter cubes <br>• Fluorescent-labeled samples <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment<br> • Different filters<br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions
+| 10:45||12:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |LMF TEAM<br>Silke||align="center" |• Light sources<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment <br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions <br> • diagramms for drawing filter/fluorophore spectra
 |-
-| ||||||||||||
+|-
+| 12:15||13:15||align="center"|SESSION 2<br>Practice||align="center" | Fluorescence imaging at microscopes ||align="center" |LMF TEAM||align="center" |- ||align="center" |• Fluorescent labeled samples
 |-
 |-
@@ Line 212: / Line 199: @@
 |align="center" style="background:DarkKhaki;"|'''LUNCH'''|| align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
 |-
-| ||||||||||||
 |-
 | 14:15||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD and Quantitative Imaging ||align="center" |LMF TEAM<br> Britta/Dan||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • RGB Slider Spot Camera setup
@@ Line 221: / Line 207: @@
 |align="center" style="background:DarkKhaki;"|'''BREAK'''|| style="background:DarkKhaki;"|'''     '''|| style="background:DarkKhaki;"|'''     '''|| style="background:DarkKhaki;"|'''     '''
 |-
-| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM<br>Jan / Peter / Dan / Britta / Silke (*)||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM <br>• Fluorescence Sample slides (Kidney / Cells
+| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM ||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes: <br> DVcore(Dan, 3 students)<br> pDV (Silke, 2 students)<br> Inverted CCD (Pete, 2 students)<br> N-Storm (Britta, 2 students) <br>self-built-syst (Jan, 3 students)) <br>• Fluorescence Sample slides (Kidney / Cells)
+|-
+|-
+| style="background:SkyBlue;"|'''19:00'''
+| style="background:SkyBlue;"|'''open end'''
+|align="center" style="background:SkyBlue;"|'''Dinner'''
+|align="center" style="background:SkyBlue;"|'''with Peter'''|| align="center" style="background:SkyBlue;"|'''LMF TEAM'''|| style="background:SkyBlue;"|'''     '''|| style="background:SkyBlue;"|'''     '''
 |}
 ''
@@ Line 235: / Line 227: @@
 | align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 |-
-| 9:00||9:30||align="center"|Recap ||align="center" |  day 4  ||align="center" |LMF TEAM
+| 9:00||9:45||align="center"|Recap ||align="center" |  day 4  ||align="center" |LMF TEAM
 |-
-| 9:30||10:20||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
+| 9:45||10:45||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 |-
 |-
-| style="background:DarkKhaki;"|'''10:20'''
+| style="background:DarkKhaki;"|'''10:45'''
-| style="background:DarkKhaki;"|'''10:35'''
+| style="background:DarkKhaki;"|'''11:00'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
 |-
-| 10:35||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
+| 11:00||13:15||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 |-
 |-
-| style="background:DarkKhaki;"|'''13:00'''
+| style="background:DarkKhaki;"|'''13:15'''
-| style="background:DarkKhaki;"|'''14:00'''
+| style="background:DarkKhaki;"|'''14:15'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''     '''
 |-
 |-
-| 14:00||15:15||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> (Own Projects) ||align="center" |LMF TEAM<br>Dan/Jan/Silke/Britta/Pete?
+| 14:15||15:00||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> (Own Projects) ||align="center" |LMF TEAM<br>Dan/Jan/Silke/Britta/Pete?
 |-
+|-
+| style="background:DarkKhaki;"|'''15:00'''
 | style="background:DarkKhaki;"|'''15:15'''
-| style="background:DarkKhaki;"|'''15:30'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''
 |align="center" style="background:DarkKhaki;"|'''BREAK'''|| align="center" style="background:DarkKhaki;"|'''BREAK'''
 |-
 |-
-| 15:30||16:30||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
+| 15:15||16:00||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
 |}
 === OFFICE DUTIES===
 ==== - LMF Members available for users (16th May to 20th May) - ====
-'''FIX ME!!!'''
+'''No office duty this time due to lack of staff. One member of the LMF will always carry the helpline phone, though.'''
 {| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
@@ Line 282: / Line 275: @@
 | |||||||| ||||
 |-
-| 9:30||12:30||align="center"| - ||align="center" | Silke ||align="center" |Jan ||align="center" |Jan ||align="center" |Britta
+| 9:30||12:30||align="center"| - ||align="center" | --- ||align="center" |--- ||align="center" |--- ||align="center" |---
 |-
 | style="background:DarkKhaki;"|'''12:30'''
@@ Line 291: / Line 284: @@
 | ||||||||||||
 |-
-| 13:30||18:00||align="center"|Britta|| align="center"|Dan ||align="center" |Dan ||align="center" |Silke||align="center" |-
+| 13:30||18:00||align="center"|---|| align="center"|--- ||align="center" |--- ||align="center" |---||align="center" |-
 |}