BasicsCoursePhDprog-PeterEvennetMay2011
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Revision as of 14:56, 11 April 2011
Contents |
PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011
Setup -Fri 13 May
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (16th May)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:00 | 09:30 | FINAL PREP | Test everything works | LMF TEAM |
9:30 | 11:05 | INTRODUCTION | COURSE INTRO WHO is WHO? Round table |
LMF TEAM |
11:05 | 11:20 | BREAK | BREAK | BREAK |
11:20 | 13:00 | SESSION 1 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses Lens aberrations Magnification Numerical aperture Microscope illumination Conjugate planes Koehler illumination |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | LUNCH | LUNCH | LUNCH |
14:00 | 15:30 | SESSION 2 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY Demo |
PETER EVENNETT LMF TEAM |
15:30 | 15:45 | BREAK | BREAK | BREAK |
15:45 | 18:00 | SESSION 3 Theory & Practice |
PRINCIPLES OF LIGHT MICROSCOPY | PETER EVENNETT LMF TEAM |
DAY TWO (May 17th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:00 | 9:30 | Recap | day 1 | LMF TEAM |
9:30 | 10:45 | SESSION 1 Theory & Practice |
Resolution Diffraction |
PETER EVENNETT LMF TEAM |
10:45 | 11:00 | BREAK | COFFEE BREAK | |
11:00 | 13:00 | DEMONSTRATION | Abbe´s diffraction experiments |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | BREAK | LUNCH | |
14:00 | 16:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
16:00 | 16:15 | BREAK | COFFEE BREAK | |
16:15 | 18:00 | SESSION 2 Theory & Practice |
Bright Field Dark Field Phase Contrast microscopy |
PETER EVENNETT LMF TEAM |
DAY THREE (May 18th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:00 | 9:30 | Recap | day 2 | LMF TEAM |
9:30 | 11:30 | SESSION 1 Theory & Practice |
Objective reading Objective cleaning |
PETER EVENNETT LMF TEAM |
11:30 | 11:45 | BREAK | BREAK | BREAK |
11:45 | 13:00 | SESSION 2 Theory & Practice |
Polarized light microscopy Differential Interference Contrast (DIC) |
PETER EVENNETT LMF TEAM |
13:00 | 14:00 | BREAK | LUNCH | |
14:00 | 15:15 | SESSION 3 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
15:15 | 15:30 | BREAK | BREAK | BREAK |
15:30 | 18:00 | SESSION 4 Theory & Practice |
Differential Interference Contrast (DIC) Objective cleaning Digital photomicrography Introduction to Confocal Microscopy |
PETER EVENNETT LMF TEAM |
DAY FOUR (May 19th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | DEMO materials |
9:00 | 9:30 | Recap | day 3 | LMF TEAM | ||
9:30 | 10:20 | SESSION 1 Theory |
Question session Fundamentals concepts of fluorescence |
LMF TEAM Dan |
- | - |
10:20 | 10:55 | BREAK | BREAK | BREAK | BREAK | BREAK |
10:55 | 13:15 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | LMF TEAM Dan? Sike? Jan? |
• Mercury/Xenon/MetalHalide lamp • Lamp houses • Spectra • Filters • Filter cubes • Fluorescent-labeled samples • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Different filters • Four (4) different Filter-sets (cubes), with the appropriate descriptions |
13:15 | 14:15 | BREAK | LUNCH | LUNCH | LUNCH | LUNCH |
14:15 | 15:45 | SESSION 3 Theory & Practice |
Imaging with CCD and Quantitative Imaging | LMF TEAM Britta/Dan |
• CCD • Cameras |
• CCD chip • RGB Slider Spot Camera setup |
15:45 | 16:00 | BREAK | BREAK | |||
16:00 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM Jan / Peter / Dan / Britta / Silke (*) |
• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM • Fluorescence Sample slides (Kidney / Cells |
DAY FIVE (May 20th)
FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
9:00 | 9:30 | Recap | day 4 | LMF TEAM |
9:30 | 10:20 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
10:20 | 10:35 | BREAK | BREAK | BREAK |
10:35 | 13:00 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
13:00 | 14:00 | BREAK | LUNCH | |
14:00 | 15:15 | SESSION 2 Small Groups Discussion |
What techniques might be good for me (Own Projects) |
LMF TEAM Dan/Jan/Silke/Britta/Pete? |
15:15 | 15:30 | BREAK | BREAK | BREAK |
15:30 | 16:30 | SESSION 3 | Course evaluation | LMF TEAM |
OFFICE DUTIES
- LMF Members available for users (16th May to 20th May) -
FIX ME!!!
FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
9:30 | 12:30 | - | Silke | Jan | Jan | Britta |
12:30 | 13:30 | L | U | N | C | H |
13:30 | 18:00 | Britta | Dan | Dan | Silke | - |