CO1 - Olympus Fluoview 1000

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m (facility code added to system number for asc *trial*)
(changes to objectives)
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| applications        = ablation experiments
 
| applications        = ablation experiments
 
| microscope          = inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC  
 
| microscope          = inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC  
| objectives          = 10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>60x/1.35 Oil UPlanSApo<br>60x/1.2 W UPlanApo
+
| objectives          = 10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>40x/1.35 UPlanSApo<br>(60x/1.35 Oil UPlanSApo - for repair)<br>60x/1.25 Oil UPlanFl - as a substitute<br>60x/1.2 W UPlanApo
 
| illumination        = Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br>
 
| illumination        = Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br>
 
| detection            = point scanner, 4 confocal [http://en.wikipedia.org/wiki/Photomultiplier PMTs], two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNt00W82g1G1TA dichromatic mirrors for excitation/emission splitting], one pinhole  
 
| detection            = point scanner, 4 confocal [http://en.wikipedia.org/wiki/Photomultiplier PMTs], two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNt00W82g1G1TA dichromatic mirrors for excitation/emission splitting], one pinhole  

Revision as of 10:45, 19 March 2009

Suitable for ablation experiments.
MPI09.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
illumination
detection [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]]
reflectors [[reflectors::Filter Cube 1. U-MWU2. BP330-385 BA420 DM400

Filter Cube 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500
Filter Cube 3. U-MWG2. BP510-550 BA590 DM570]]

features Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation"Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation" cannot be used as a page name in this wiki.
software
incubation -
links
  • [ manual]
  • [LPM laser power values]
inv.nr. -



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