CO1 - Olympus Fluoview 1000
From BioDIP
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MPI09 |
| − | | system-name | + | |system-name=Olympus Fluoview 1000 |
| − | | category | + | |type=Microscope |
| − | + | |category=Laser Scanning Confocal | |
| − | + | |facility=MPI-CBG LMF | |
| − | | facility | + | |building=MPI-CBG |
| − | + | |room=038A | |
| − | | building | + | |facility_image=logo-lmf.jpg |
| − | | room | + | |description=Single photon point scanning [[Olympus FV1000]] [[confocal]] microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser |
| − | | description | + | |applications=ablation experiments |
| − | | applications | + | |image=MPI09.jpg |
| − | | microscope | + | |stand=Inverted |
| − | | objectives | + | |microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC |
| − | | illumination | + | |objectives=10x/0.4 UPlanApo<br>20x/0.7 UPlanApo<br>40x/0.85 UPlanApo<br>40x/1.35 UPlanSApo<br>(60x/1.35 Oil UPlanSApo - for repair)<br>60x/1.25 Oil UPlanFl - as a substitute<br>60x/1.2 W UPlanApo |
| − | | detection | + | |illumination=Halogen<br>HBO<br>405nm diode laser<br>458, 488, 515 ArgonLaser<br>633 nm HeNe laser<br>561 nm Diode laser<br> |
| − | | reflectors | + | |detection=point scanner, 4 confocal [http://en.wikipedia.org/wiki/Photomultiplier PMTs], two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNt00W82g1G1TA dichromatic mirrors for excitation/emission splitting], one pinhole |
| + | |reflectors=Filter Cube 1. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwu2.asp U-MWU2]. BP330-385 BA420 DM400 | ||
Filter Cube 2. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter) DM500<br> | Filter Cube 2. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter) DM500<br> | ||
Filter Cube 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550 BA590 DM570 | Filter Cube 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550 BA590 DM570 | ||
| − | | features | + | |features=Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation |
| − | | incubation | + | |incubation=stage incubator (T+CO2) |
| − | | link1 | + | |link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSmt7IA5ZOd9Yw LPM] |
| − | | link2 | + | |link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNu6qJfppRd3JQ scan-head configuration] |
| − | | inv | + | |inv=- |
| + | |obj1=- | ||
| + | |obj2=- | ||
| + | |obj3=- | ||
| + | |obj4=- | ||
| + | |obj5=- | ||
| + | |obj6=- | ||
| + | |obj7=- | ||
| + | |obj8=- | ||
}} | }} | ||
Revision as of 17:12, 1 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination |
| ||
| detection | [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]] | ||
| reflectors | [[reflectors::Filter Cube 1. U-MWU2. BP330-385 BA420 DM400
Filter Cube 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500 | ||
| features | Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation"Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation" cannot be used as a page name in this wiki. | ||
| software | |||
| incubation | stage incubator (T+CO2) | ||
| links |
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| inv.nr. | - |
