Chromatic aberration measurement and correction
From BioDIP
(Difference between revisions)
(Created page with '*overlay UV/V and VIS (confocal systems with separate UV/V laser coupling) **purpose: check the coupling precision of the UV/V laser **requirements: 0.2/0.5µm fluorescent beads ...') |
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**requirements: 0.2/0.5µm fluorescent beads sample | **requirements: 0.2/0.5µm fluorescent beads sample | ||
**work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm) | **work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm) | ||
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Revision as of 14:19, 30 September 2009
- overlay UV/V and VIS (confocal systems with separate UV/V laser coupling)
- purpose: check the coupling precision of the UV/V laser
- requirements: 0.2/0.5µm fluorescent beads sample
- work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm)
