Chromatic aberration measurement and correction
From BioDIP
(Difference between revisions)
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**requirements: 0.2/0.5µm fluorescent beads sample | **requirements: 0.2/0.5µm fluorescent beads sample | ||
**work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm) | **work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm) | ||
| + | == images == | ||
| + | <gallery> | ||
| + | file:Coreg XY line profiles fiji.jpg|quick & dirty coregistration analysis in Fiji, 200 nm beads, 2 line profiles for "blue" and "green" | ||
| + | </gallery> | ||
[[category:ASC]] | [[category:ASC]] | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 10:32, 1 October 2009
- overlay UV/V and VIS (confocal systems with separate UV/V laser coupling)
- purpose: check the coupling precision of the UV/V laser
- requirements: 0.2/0.5µm fluorescent beads sample
- work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm)
images
quick & dirty coregistration analysis in Fiji, 200 nm beads, 2 line profiles for "blue" and "green"
