Chromatic aberration measurement and correction

From BioDIP
Revision as of 09:51, 1 October 2009 by Old user (Talk | contribs)

Jump to: navigation, search
  • overlay UV/V and VIS (confocal systems with separate UV/V laser coupling)
    • purpose: check the coupling precision of the UV/V laser
    • requirements: 0.2/0.5µm fluorescent beads sample
    • work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm)
Personal tools