D1 - SPIM Demo
From BioDIP
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{{Equipment | {{Equipment | ||
| − | |system-number= | + | |system-number=MPI-D1 |
| − | |system-name=SPIM | + | |system-name=Zeiss SPIM Demonstrator B |
| − | |type= | + | |type=Not listed |
|category=Single Plane Illumination | |category=Single Plane Illumination | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
| − | |room=- | + | |room=-341 |
| − | + | |description=This Zeiss demo version of a single plane illumination system allows the acquisition of 3D image data from large and small 3D mounted, live or fixed specimens. | |
| − | |description=This demo version of a single plane illumination system allows the acquisition of 3D image data from | + | |applications=developmental studies on living embryos, spheroids, cleared tissues, etc. |
| − | |applications=developmental studies on living embryos | + | |
|image=MPI00.jpg | |image=MPI00.jpg | ||
| + | |stand-name=Zeiss prototype SPIM 3D stand | ||
|stand=3D | |stand=3D | ||
| − | |microscope=imaging chamber, light sheet illumination | + | |microscope=imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) |
| − | | | + | |obj1=Zeiss W N-Achroplan 20x/0.5 |
| − | | | + | |obj2=Zeiss N-Achroplan 63x 0.9 W |
| − | |detection=Zeiss CCD camera | + | |ill1=Transmitted Light (LED) |
| − | |reflectors=- | + | |ill2=Fluorescence (HBO) |
| − | |features= | + | |ill3=Laser Argon Multiline 458, 477, 488, 514 nm |
| − | |incubation= | + | |ill4=Laser DPSS 561 nm |
| + | |ill5=Laser HeNe 633 nm | ||
| + | |detection=Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning. | ||
| + | |reflectors=50/50 for sample location/lightsheet alignment etc., | ||
| + | BrightLine HC FITC F36-501, | ||
| + | Green Fset09, | ||
| + | BrightLine HC mCherry 624/40 F37-624 emitter only, | ||
| + | YFP FSet46he, | ||
| + | CFP FSet51, | ||
| + | BrightLine HC Green 514/30, | ||
| + | |features=Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. | ||
| + | It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. | ||
| + | No 405/UV spim illumination sorry. | ||
| + | |software=Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| + | |incubation=Stage incubator (T) | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
| − | | | + | |inv=0 10.1.235.28 |
| − | | | + | |facility_image=logo-lmf.jpg |
| − | | | + | |objectives=water dipping and low mag air lenses |
| − | + | |illumination=LED<br>X-Cite<br>Argon laser | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | | | + | |
}} | }} | ||
Latest revision as of 10:52, 5 November 2013
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[edit] Directions
[[image:|150px|text-bottom|right]]
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Zeiss prototype SPIM 3D stand, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
| objectives | |||
| illumination | |||
| detection | Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning. | ||
| reflectors | 50/50 for sample location/lightsheet alignment etc.,
BrightLine HC FITC F36-501, Green Fset09, BrightLine HC mCherry 624/40 F37-624 emitter only, YFP FSet46he, CFP FSet51, BrightLine HC Green 514/30, | ||
| features | Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium.
It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki. | ||
| software | Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| incubation | Stage incubator (T) | ||
| links |
| ||
| inv.nr. | 0 10.1.235.28 |
