D1 - SPIM Demo
From BioDIP
(Difference between revisions)
m (moved SPIM Demo to D1 - SPIM Demo: match booking database) |
|||
| Line 6: | Line 6: | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
| − | |room=- | + | |room=-341 |
|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
|description=This demo version of a single plane illumination system allows the acquisition of 3D image data from thick specimens. | |description=This demo version of a single plane illumination system allows the acquisition of 3D image data from thick specimens. | ||
|applications=developmental studies on living embryos | |applications=developmental studies on living embryos | ||
|image=MPI00.jpg | |image=MPI00.jpg | ||
| + | |stand-name=Leica - DMRE | ||
|stand=3D | |stand=3D | ||
| − | |microscope=imaging chamber, light sheet illumination | + | |microscope=imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) |
| + | |obj1=Zeiss W N-Achroplan 20x/0.5 | ||
| + | |obj2=Zeiss N-Achroplan 63x 0.9 W | ||
|ill1=Transmitted Light (LED) | |ill1=Transmitted Light (LED) | ||
| − | |ill2=Fluorescence ( | + | |ill2=Fluorescence (HBO) |
|ill3=Laser Argon Multiline 458, 477, 488, 514 nm | |ill3=Laser Argon Multiline 458, 477, 488, 514 nm | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
| − | |detection=Zeiss CCD camera | + | |ill5=Laser HeNe 633 nm |
| − | |reflectors=- | + | |detection=Zeiss CCD axiocam camera |
| − | |features= | + | |reflectors=50/50 for sample location/lightsheet alignment etc. |
| − | |incubation= | + | BrightLine HC FITC F36-501 |
| + | Green Fset09 | ||
| + | BrightLine HC mCherry 624/40 F37-624 emitter only | ||
| + | YFP FSet46he | ||
| + | CFP FSet51 | ||
| + | BrightLine HC Green 514/30 | ||
| + | |features=Its got a lightsheet and perpendicular detection. | ||
| + | No 405/UV spim illumination sorry. | ||
| + | |software=Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| + | |incubation=Stage incubator (T) | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
Revision as of 13:15, 7 June 2010
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Leica - DMRE, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
| objectives | |||
| illumination | |||
| detection | Zeiss CCD axiocam camera | ||
| reflectors | 50/50 for sample location/lightsheet alignment etc.
BrightLine HC FITC F36-501 Green Fset09 BrightLine HC mCherry 624/40 F37-624 emitter only YFP FSet46he CFP FSet51 BrightLine HC Green 514/30 | ||
| features | Its got a lightsheet and perpendicular detection.
No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki. | ||
| software | Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| incubation | Stage incubator (T) | ||
| links |
| ||
| inv.nr. | 0 |
