D1 - SPIM Demo
From BioDIP
(Difference between revisions)
| Line 11: | Line 11: | ||
|applications=developmental studies on living embryos | |applications=developmental studies on living embryos | ||
|image=MPI00.jpg | |image=MPI00.jpg | ||
| − | |stand-name= | + | |stand-name=Leica - DMRE |
|stand=3D | |stand=3D | ||
| − | |microscope=imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | + | |microscope=imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) |
|obj1=Zeiss W N-Achroplan 20x/0.5 | |obj1=Zeiss W N-Achroplan 20x/0.5 | ||
|obj2=Zeiss N-Achroplan 63x 0.9 W | |obj2=Zeiss N-Achroplan 63x 0.9 W | ||
| Line 22: | Line 22: | ||
|ill5=Laser HeNe 633 nm | |ill5=Laser HeNe 633 nm | ||
|detection=Zeiss CCD axiocam camera | |detection=Zeiss CCD axiocam camera | ||
| − | |reflectors=50/50 for sample location/lightsheet alignment etc. | + | |reflectors=50/50 for sample location/lightsheet alignment etc., |
| − | BrightLine HC FITC F36-501 | + | BrightLine HC FITC F36-501, |
| − | Green Fset09 | + | Green Fset09, |
| − | BrightLine HC mCherry 624/40 F37-624 emitter only | + | BrightLine HC mCherry 624/40 F37-624 emitter only, |
| − | YFP FSet46he | + | YFP FSet46he, |
| − | CFP FSet51 | + | CFP FSet51, |
| − | BrightLine HC Green 514/30 | + | BrightLine HC Green 514/30, |
|features=Its got a lightsheet and perpendicular detection. | |features=Its got a lightsheet and perpendicular detection. | ||
| − | No 405/UV spim illumination sorry. | + | No 405/UV spim illumination sorry. |
| − | |software=Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | + | |software=Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. |
|incubation=Stage incubator (T) | |incubation=Stage incubator (T) | ||
|link1=- | |link1=- | ||
Revision as of 13:16, 7 June 2010
|
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Leica - DMRE, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
| objectives | |||
| illumination | |||
| detection | Zeiss CCD axiocam camera | ||
| reflectors | 50/50 for sample location/lightsheet alignment etc.,
BrightLine HC FITC F36-501, Green Fset09, BrightLine HC mCherry 624/40 F37-624 emitter only, YFP FSet46he, CFP FSet51, BrightLine HC Green 514/30, | ||
| features | Its got a lightsheet and perpendicular detection.
No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki. | ||
| software | Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| incubation | Stage incubator (T) | ||
| links |
| ||
| inv.nr. | 0 |
