D1 - SPIM Demo
From BioDIP
(Difference between revisions)
| Line 8: | Line 8: | ||
|room=-341 | |room=-341 | ||
|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
| − | |description=This demo version of a single plane illumination system allows the acquisition of 3D image data from | + | |description=This Zeiss demo version of a single plane illumination system allows the acquisition of 3D image data from large and small 3D mounted, live or fixed specimens. |
| − | |applications=developmental studies on living embryos | + | |applications=developmental studies on living embryos, spheroids, cleared tissues, etc. |
|image=MPI00.jpg | |image=MPI00.jpg | ||
|stand-name=Leica - DMRE | |stand-name=Leica - DMRE | ||
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|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
|ill5=Laser HeNe 633 nm | |ill5=Laser HeNe 633 nm | ||
| − | |detection=Zeiss CCD | + | |detection=Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning. |
|reflectors=50/50 for sample location/lightsheet alignment etc., | |reflectors=50/50 for sample location/lightsheet alignment etc., | ||
BrightLine HC FITC F36-501, | BrightLine HC FITC F36-501, | ||
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CFP FSet51, | CFP FSet51, | ||
BrightLine HC Green 514/30, | BrightLine HC Green 514/30, | ||
| − | |features=Its got a lightsheet and perpendicular detection. | + | |features=Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. |
| + | It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. | ||
No 405/UV spim illumination sorry. | No 405/UV spim illumination sorry. | ||
|software=Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | |software=Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
Revision as of 13:20, 7 June 2010
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Leica - DMRE, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
| objectives | |||
| illumination | |||
| detection | Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning. | ||
| reflectors | 50/50 for sample location/lightsheet alignment etc.,
BrightLine HC FITC F36-501, Green Fset09, BrightLine HC mCherry 624/40 F37-624 emitter only, YFP FSet46he, CFP FSet51, BrightLine HC Green 514/30, | ||
| features | Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium.
It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki. | ||
| software | Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| incubation | Stage incubator (T) | ||
| links |
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| inv.nr. | 0 |
