D1 - SPIM Demo

Suitable for developmental studies on living embryos.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, -341)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Prototype Zeiss SPIM., 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x)
objectives	Zeiss W N-Achroplan 20x/0.5 Zeiss N-Achroplan 63x 0.9 W
illumination	Transmitted Light (LED) Fluorescence (HBO) Laser Argon Multiline 458, 477, 488, 514 nm Laser DPSS 561 nm Laser HeNe 633 nm
detection	Zeiss CCD axiocam camera
reflectors	50/50 for sample location/lightsheet alignment etc. BrightLine HC FITC F36-501 Green Fset09 BrightLine HC mCherry 624/40 F37-624 emitter only YFP FSet46he CFP FSet51 BrightLine HC Green 514/30
features	Its got a lightsheet and perpendicular detection. No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki.
software	Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible.
incubation	Stage incubator (T)
links	[ manual] [- laser power values] [- beam path] [ advanced system check]
inv.nr.	0

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