D1 - SPIM Demo
From BioDIP
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|building=MPI-CBG | |building=MPI-CBG | ||
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|description=This Zeiss demo version of a single plane illumination system allows the acquisition of 3D image data from large and small 3D mounted, live or fixed specimens. | |description=This Zeiss demo version of a single plane illumination system allows the acquisition of 3D image data from large and small 3D mounted, live or fixed specimens. | ||
|applications=developmental studies on living embryos, spheroids, cleared tissues, etc. | |applications=developmental studies on living embryos, spheroids, cleared tissues, etc. | ||
|image=MPI00.jpg | |image=MPI00.jpg | ||
| − | |stand-name=Zeiss | + | |stand-name=Zeiss prototype SPIM 3D stand |
|stand=3D | |stand=3D | ||
|microscope=imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | |microscope=imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
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| + | |facility_image=logo-lmf.jpg | ||
|objectives=water dipping and low mag air lenses | |objectives=water dipping and low mag air lenses | ||
|illumination=LED<br>X-Cite<br>Argon laser | |illumination=LED<br>X-Cite<br>Argon laser | ||
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Revision as of 18:19, 2 May 2011
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Zeiss prototype SPIM 3D stand, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x) | ||
| objectives | |||
| illumination | |||
| detection | Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning. | ||
| reflectors | 50/50 for sample location/lightsheet alignment etc.,
BrightLine HC FITC F36-501, Green Fset09, BrightLine HC mCherry 624/40 F37-624 emitter only, YFP FSet46he, CFP FSet51, BrightLine HC Green 514/30, | ||
| features | Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium.
It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki. | ||
| software | Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible. | ||
| incubation | Stage incubator (T) | ||
| links |
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| inv.nr. | 0 |
