H1 - DeltaVision OMX - pDV
From BioDIP
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI-H1 | + | |system-number=MPI- H1 |
|system-name=DeltaVision OMX - pDV | |system-name=DeltaVision OMX - pDV | ||
|type=Microscope | |type=Microscope | ||
| − | |category= | + | |category=Superresolution |
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=036 | |room=036 | ||
| − | |Facility-logo=MPI-CBG | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| − | |description=Structured Illumination Superresolution and fast widefield. 4x | + | |Institute-logo=Logo MPI-CBG.jpg |
| − | |applications= | + | |description=Applied Precision Inc Deltavision OMX v4 BLAZE: Live cell and fixed sample Structured Illumination Superresolution and very fast widefield. 4x sCMOS cameras, 405, 488, 568, 642 laser illumination widefield or structured 3D illumination. Also has its own personal DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide. |
| + | |applications=high resolution imaging of fixed and living samples down to 120 nm, and very fast widefield imaging. | ||
| + | |image=H1-DeltaVision OMX-pDV.JPG | ||
|stand-name=Applied Precision - OMX stand | |stand-name=Applied Precision - OMX stand | ||
|stand=Inverted | |stand=Inverted | ||
| − | |microscope=nano precise XYZ stage, fluorescence, transmitted light, DIC | + | |microscope=nano precise XYZ stage, fast z piezo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV. |
| − | |obj1=Olympus | + | |obj1=Olympus UPlanSApochromat 100x 1.4 Oil |
| + | |obj2=Olympus UPlanSApochromat 10x 0.4 | ||
| + | |obj3=Olympus PlanApochromatN 60x 1.42 Oil | ||
| + | |obj4=Olympus PlanApochromatN 60x 1.42 Oil | ||
| + | |obj5=Leica Achromatic 10x 0.25 | ||
| + | |obj6=Olympus PlanApochromatN 60x 1.42 Oil | ||
|ill1=Transmitted Light (LED) | |ill1=Transmitted Light (LED) | ||
|ill2=Laser Diode 405 nm | |ill2=Laser Diode 405 nm | ||
| Line 19: | Line 26: | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
|ill5=Laser DPSS 640 nm | |ill5=Laser DPSS 640 nm | ||
| − | |detection=4x | + | |detection=4x sCMOS cooled to 3oC. Simultaneous or sequential imaging. |
| − | |reflectors=standard set, OMX. See Docs at OMX. | + | |reflectors="standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set; second set for CFP/YFP/RFP |
| − | |features=fast 4 colour widefield. Super resolution by structured illumination in 3D, down to | + | |features=fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy) |
|software=API softworx and OMX software for driving the scope and super resolution reconstruction. | |software=API softworx and OMX software for driving the scope and super resolution reconstruction. | ||
| + | OMX SI reconstruction linux box: centos 4, 10.1.145.31 | ||
| + | personalDV linux centos5 box 10.1.145.32 | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
| + | |link0=[H1_OMX_Startup_and_Shutdown_manual]] | ||
| + | |link1=https://docs.google.com/spreadsheets/d/1azxZb1ZJFiPVnBz2gU-vSto4lPvairiyG9_LtmCSokU/pubhtml | ||
|inv=MOZG040809900 | |inv=MOZG040809900 | ||
|facility_image=Logo-lmf.jpg | |facility_image=Logo-lmf.jpg | ||
| Line 29: | Line 40: | ||
|illumination=Halogen<br>HBO<br>458, 477, 488, 514 nm Argon laser<br>543 nm HeNe laser<br>633 nm HeNe laser | |illumination=Halogen<br>HBO<br>458, 477, 488, 514 nm Argon laser<br>543 nm HeNe laser<br>633 nm HeNe laser | ||
}} | }} | ||
| + | [[category:OMX]] | ||
Latest revision as of 15:23, 8 November 2016
|
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Applied Precision - OMX stand, Inverted stand, nano precise XYZ stage, fast z piezo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV. | ||
| objectives | |||
| illumination | |||
| detection | 4x sCMOS cooled to 3oC. Simultaneous or sequential imaging. | ||
| reflectors | "standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set; second set for CFP/YFP/RFP | ||
| features | fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy) | ||
| software | API softworx and OMX software for driving the scope and super resolution reconstruction.
OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32"API softworx and OMX software for driving the scope and super resolution reconstruction. OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32" cannot be used as a page name in this wiki. | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. | MOZG040809900 |
