View source for H2 - Nikon STORM - TIRF

{{Equipment
|system-number=MPI- H2
|system-name=Nikon STORM - TIRF
|type=Microscope
|category=Superresolution
|facility=MPI-CBG LMF
|building=MPI-CBG
|room=036
|Facility-logo=MPI-CBG-Lmf-logo-big.png
|Institute-logo=Logo MPI-CBG.jpg
|description=Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;
|applications=N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging
|image=H2 - Nikon STORM - TIRF.jpg
|stand-name=Nikon - Eclipse Ti
|stand=Inverted
|microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br>motorized TIRF-condenser with additional manual collimating lens
|obj1=Nikon Plan-Apochromat 10x 0.45 DIC
|obj2=Nikon Plan-ApochromatVC 60x 1.2 Water DIC
|obj3=Nikon Apochromat 60x 1.49 Oil DIC
|obj4=Nikon ApochromatVC 100x 1.4 Oil DIC
|obj5=Nikon SR-Apochromat 100x 1.49 Oil DIC
|obj6=Nikon Planfluor 20x/0.75 MImm
|ill1=Transmitted Light (Halogen)
|ill2=Fluorescence (LED)
|ill3=Laser Diode 405 nm
|ill4=Laser DPSS 488 nm
|ill5=Laser DPSS 561 nm
|ill6=Laser DPSS 640 nm
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD SN #4431 with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD # 6499 with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
|reflectors=Microscope stand:<br>reflector revolver FL2 (lower floor):<br>position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: Dual LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 4: narrowGFP (only EM 510/40), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty<br> reflector revolver FL1 (upper floor):<br> position 1: TIRF1 cube (quad DC: zt405/488/561/640rpc +  quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 2: TIRF2Q cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 5: Analyzer, 6: empty <br>Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15  <br>Hamamatsu W-View image splitter:<br>for bypass mode: silver mirror<br>for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40
|features=-
|software=NIS Elements 4.51.01 with N-STORM module + Jobs module
|incubation=Cage incubator (T+CO2)
|link1=https://docs.google.com/spreadsheets/d/1XQ6Yf8XW73ykHZhnGgAN7ZPOq0zKcXlFIW1DgkPbbBc/pubhtml
|inv=0  10.1.32.156
}}