H2 - Nikon STORM - TIRF
From BioDIP
(Difference between revisions)
| Line 15: | Line 15: | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br>motorized TIRF-condenser with additional manual collimating lens | |microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br>motorized TIRF-condenser with additional manual collimating lens | ||
| − | |obj1=Nikon | + | |obj1=Nikon Plan-Apochromat 10x 0.45 DIC |
| − | |obj2=Nikon | + | |obj2=Nikon Plan-ApochromatVC 60x 1.2 Water DIC |
|obj3=Nikon Apochromat 60x 1.49 Oil DIC | |obj3=Nikon Apochromat 60x 1.49 Oil DIC | ||
|obj4=Nikon ApochromatVC 100x 1.4 Oil DIC | |obj4=Nikon ApochromatVC 100x 1.4 Oil DIC | ||
| − | |obj5=Nikon | + | |obj5=Nikon SR-Apochromat 100x 1.49 Oil DIC |
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (LED) | |ill2=Fluorescence (LED) | ||
Revision as of 15:34, 29 October 2014
|
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki. | ||
| objectives | |||
| illumination | |||
| detection | at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
| reflectors | Microscope stand: reflector revolver FL1 (lower floor): position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty reflector revolver FL2 (upper floor): position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28 , 3: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60) , 4: YFP HQ cube (EX: ET 470/40, DC: 496LP, EM: 537/42), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty Sutter filter wheel: position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 Hamamatsu W-View image splitter: for bypass mode: silver mirror for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 | ||
| features | - | ||
| software | NIS Elements with N-STORM module | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 0 10.1.32.156 |
