H2 - Nikon STORM - TIRF

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{{Equipment
 
{{Equipment
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|system-number=MPI- W1
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|system-number=MPI- H2
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|system-name=Olympus TIRF
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|system-name=Nikon STORM - TIRF
 
|type=Microscope
 
|type=Microscope
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|category=Total Internal Reflection
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|category=Superresolution
 
|facility=MPI-CBG LMF
 
|facility=MPI-CBG LMF
 
|building=MPI-CBG
 
|building=MPI-CBG
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|room=340
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|room=036
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|description=Olympus TIRF setup.
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|Facility-logo=MPI-CBG-Lmf-logo-big.png
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|applications=membrane trafficking studies
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|Institute-logo=Logo MPI-CBG.jpg
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|image=MPI_W1_TIRF.jpg
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|description=Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;
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|stand-name=Olympus - IX71
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|applications=N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging
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|image=H2 - Nikon STORM - TIRF.jpg
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|stand-name=Nikon - Eclipse Ti
 
|stand=Inverted
 
|stand=Inverted
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|microscope=manual, manual XY stage
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|microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br>motorized TIRF-condenser with additional manual collimating lens
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|obj1=Olympus UPlanSApo 10x 0.4
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|obj1=Nikon Plan-Apochromat 10x 0.45 DIC
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|obj2=Olympus PlanApo 100x 1.45 TIRFM
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|obj2=Nikon Plan-ApochromatVC 60x 1.2 Water DIC
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|obj3=Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr
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|obj3=Nikon Apochromat 60x 1.49 Oil DIC
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|obj4=Nikon ApochromatVC 100x 1.4 Oil DIC
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|obj5=Nikon SR-Apochromat 100x 1.49 Oil DIC
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|obj6=Nikon Planfluor 20x/0.75 MImm
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
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|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
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|ill2=Fluorescence (LED)
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|ill3=Laser DPSS 491 nm
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|ill3=Laser Diode 405 nm
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|ill4=Laser DPSS 561 nm
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|ill4=Laser DPSS 488 nm
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|ill5=Laser DPSS 640 nm
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|ill5=Laser DPSS 561 nm
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|detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
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|ill6=Laser DPSS 640 nm
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image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um;
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|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD SN #4431 with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD # 6499 with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
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with 150x objective: 0.106um (measured)
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|reflectors=Microscope stand:<br>reflector revolver FL2 (lower floor):<br>position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: Dual LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 4: narrowGFP (only EM 510/40), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty<br> reflector revolver FL1 (upper floor):<br> position 1: TIRF1 cube (quad DC: zt405/488/561/640rpc + quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 2: TIRF2Q cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 5: Analyzer, 6: empty <br>Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 <br>Hamamatsu W-View image splitter:<br>for bypass mode: silver mirror<br>for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40
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|reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40<br>Sutter filter wheel: <br>position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: EdgeBasic 635 LP,  8: BL HC LP488, 9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!,  5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
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|features=-
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|features=custom-built TIRF condenser, manual TIRF angle adjustment
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|software=NIS Elements 4.51.01 with N-STORM module + Jobs module
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|software=iQ 1.9.1
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|incubation=Cage incubator (T+CO2)
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|incubation=Stage incubator (T+CO2) + objective heater
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|link1=https://docs.google.com/spreadsheets/d/1XQ6Yf8XW73ykHZhnGgAN7ZPOq0zKcXlFIW1DgkPbbBc/pubhtml
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|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA
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|inv=0  10.1.32.156
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|inv=404504
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|facility_image=logo-lmf.jpg
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|objectives=PlanApo 100x / 1.45 Oil TIRFM
+
−
|illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt
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}}
 
}}

Latest revision as of 16:33, 23 November 2016

Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.
H2 - Nikon STORM - TIRF.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 036)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;
motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki.
objectives
Nikon Plan-Apochromat 10x 0.45 DIC
Nikon Plan-ApochromatVC 60x 1.2 Water DIC
Nikon Apochromat 60x 1.49 Oil DIC
Nikon ApochromatVC 100x 1.4 Oil DIC
Nikon SR-Apochromat 100x 1.49 Oil DIC
Nikon Planfluor 20x/0.75 MImm
illumination
Transmitted Light (Halogen)
Fluorescence (LED)
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD SN #4431 with cylindrical lens; dexel size of camera chip: 16um
at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD # 6499 with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
reflectors Microscope stand:
reflector revolver FL2 (lower floor):
position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: Dual LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 4: narrowGFP (only EM 510/40), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty
reflector revolver FL1 (upper floor):
position 1: TIRF1 cube (quad DC: zt405/488/561/640rpc + quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 2: TIRF2Q cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 5: Analyzer, 6: empty
Sutter filter wheel:
position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15
Hamamatsu W-View image splitter:
for bypass mode: silver mirror
for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40
features -
software NIS Elements 4.51.01 with N-STORM module + Jobs module
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 0 10.1.32.156


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