H2 - Nikon STORM - TIRF
From BioDIP
(Difference between revisions)
| Line 22: | Line 22: | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (LED) | |ill2=Fluorescence (LED) | ||
| − | |ill3=Laser DPSS 488 nm | + | |ill3=Laser Diode 405 nm |
| − | | | + | |ill4=Laser DPSS 488 nm |
| − | | | + | |ill5=Laser DPSS 561 nm |
| + | |ill6=Laser DPSS 640 nm | ||
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | |detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
|reflectors=Microscope stand:<br>reflector revolver FL1 (lower floor):<br>position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty<br> reflector revolver FL2 (upper floor):<br> position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty <br>Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 <br>Hamamatsu W-View image splitter:<br>for bypass mode: silver mirror<br>for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 | |reflectors=Microscope stand:<br>reflector revolver FL1 (lower floor):<br>position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty<br> reflector revolver FL2 (upper floor):<br> position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty <br>Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 <br>Hamamatsu W-View image splitter:<br>for bypass mode: silver mirror<br>for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 | ||
|features=- | |features=- | ||
| − | |software=NIS Elements with N-STORM module | + | |software=NIS Elements with N-STORM module + Jobs module |
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
|link1=https://docs.google.com/spreadsheets/d/1XQ6Yf8XW73ykHZhnGgAN7ZPOq0zKcXlFIW1DgkPbbBc/pubhtml | |link1=https://docs.google.com/spreadsheets/d/1XQ6Yf8XW73ykHZhnGgAN7ZPOq0zKcXlFIW1DgkPbbBc/pubhtml | ||
|inv=0 10.1.32.156 | |inv=0 10.1.32.156 | ||
}} | }} | ||
Revision as of 11:20, 22 November 2016
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Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki. | ||
| objectives | |||
| illumination | |||
| detection | at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
| reflectors | Microscope stand: reflector revolver FL1 (lower floor): position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty reflector revolver FL2 (upper floor): position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty Sutter filter wheel: position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 Hamamatsu W-View image splitter: for bypass mode: silver mirror for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 | ||
| features | - | ||
| software | NIS Elements with N-STORM module + Jobs module | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 0 10.1.32.156 |
