H2 - Nikon STORM - TIRF
From BioDIP
(Difference between revisions)
| Line 10: | Line 10: | ||
|applications=membrane trafficking studies | |applications=membrane trafficking studies | ||
|image=MPI_W1_TIRF.jpg | |image=MPI_W1_TIRF.jpg | ||
| − | |stand-name=Nikon - Eclipse | + | |stand-name=Nikon - Eclipse Ti |
|stand=Inverted | |stand=Inverted | ||
|microscope=manual, manual XY stage | |microscope=manual, manual XY stage | ||
Revision as of 18:31, 2 May 2011
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Nikon - Eclipse Ti, Inverted stand, manual, manual XY stage | ||
| objectives | |||
| illumination | |||
| detection | Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um; with 150x objective: 0.106um (measured) | ||
| reflectors | Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40 Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: EdgeBasic 635 LP, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575 | ||
| features | custom-built TIRF condenser, manual TIRF angle adjustment | ||
| software | iQ 1.9.1 | ||
| incubation | Stage incubator (T+CO2) + objective heater | ||
| links |
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| inv.nr. | 404504 |
