H2 - Nikon STORM - TIRF

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|building=MPI-CBG
 
|building=MPI-CBG
 
|room=340
 
|room=340
−
|description=Olympus TIRF setup.
+
|description=nikon TIRF / N-STORM setup.
−
|applications=membrane trafficking studies
+
|applications=N-STORM, fast TIRF
−
|image=MPI_W1_TIRF.jpg
+
 
|stand-name=Nikon - Eclipse Ti
 
|stand-name=Nikon - Eclipse Ti
 
|stand=Inverted
 
|stand=Inverted
−
|microscope=manual, manual XY stage
+
|microscope=motorised stage
−
|obj1=Olympus UPlanSApo 10x 0.4
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|obj1=Nikon PlanApo 60x/1.2 WI 0.15-0.18
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|obj2=Olympus PlanApo 100x 1.45 TIRFM
+
−
|obj3=Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr
+
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
−
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
+
|ill2=Fluorescence (LED)
−
|ill3=Laser DPSS 491 nm
+
|ill3=Laser Argon Multiline 458, 477, 488, 514 nm
 
|ill4=Laser DPSS 561 nm
 
|ill4=Laser DPSS 561 nm
 
|ill5=Laser DPSS 640 nm
 
|ill5=Laser DPSS 640 nm
−
|detection=Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um
+
|detection=Andor EM-CCD
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image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um;
+
 
−
with 150x objective: 0.106um (measured)
+
|reflectors=image splitter:
−
|reflectors=Hamamatsu W-View image splitter:<br>two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40<br>Sutter filter wheel: <br>position 0: BL HC 520/15,  1: BL HC 628/40,  2: BL HC 525/30,  3: BL HC 607/36,  4: dual emitter: Bl HC 512/23+630/91,  5: open,  6: dual emitter:  Z491/561,  7: EdgeBasic 635 LP,  8: BL HC LP488,  9: BL HC 617/73<br> microscope filter turret:<br> position 1: for TIRF 488Ex/DC500/LP505Em,  2: for TIRF Z488Ex/DCZDC488/BP 520/40,  3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!,  5: dual Ex and DC for 491 and 561 laser excitation, no emitter!,  6: only DC and EM LP 575
+
|features=-
−
|features=custom-built TIRF condenser, manual TIRF angle adjustment
+
|software=NIS Elements
−
|software=iQ 1.9.1
+
 
|incubation=Stage incubator (T+CO2) + objective heater
 
|incubation=Stage incubator (T+CO2) + objective heater
−
|link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvcd1zCT8I_CA
+
|inv=0
−
|inv=404504
+
 
|facility_image=logo-lmf.jpg
 
|facility_image=logo-lmf.jpg
 
|objectives=PlanApo 100x / 1.45 Oil TIRFM
 
|objectives=PlanApo 100x / 1.45 Oil TIRFM
 
|illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt
 
|illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt
 
}}
 
}}

Revision as of 18:33, 2 May 2011

Suitable for N-STORM, fast TIRF.
[[image:|250px]]


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 340)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Nikon - Eclipse Ti, Inverted stand, motorised stage
objectives
Nikon PlanApo 60x/1.2 WI 0.15-0.18
illumination
Transmitted Light (Halogen)
Fluorescence (LED)
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection Andor EM-CCD
reflectors image splitter:
features -
software NIS Elements
incubation Stage incubator (T+CO2) + objective heater
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0


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