MPI-CL1 - Leica TCS SP2 - startup & shutdown
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== How to start the Leica TCS SP2 == | == How to start the Leica TCS SP2 == | ||
| − | === | + | === Control Panel === |
[[image:mpi03_panel.jpg|200x100px]] | [[image:mpi03_panel.jpg|200x100px]] | ||
# Switch buttons from right to left (PC-Scanner-Laser) | # Switch buttons from right to left (PC-Scanner-Laser) | ||
| Line 7: | Line 7: | ||
# Argon laser for laser lines: 458, 477, 488, 514. To switch on first turn the key to START, it then jumps automatically back to the ON position. | # Argon laser for laser lines: 458, 477, 488, 514. To switch on first turn the key to START, it then jumps automatically back to the ON position. | ||
# Switch on 543nm and 633nm laser line if needed. | # Switch on 543nm and 633nm laser line if needed. | ||
| − | === | + | === Software Initiation === |
[[image:mpi03_setlimit.jpg|200x100px]] [[image:mpi03_icon.jpg|200x100px]] [[image:mpi03_software.jpg|200x100px]] | [[image:mpi03_setlimit.jpg|200x100px]] [[image:mpi03_icon.jpg|200x100px]] [[image:mpi03_software.jpg|200x100px]] | ||
# Make sure that the upper stage limit is set before starting the Leica Confocal Software. | # Make sure that the upper stage limit is set before starting the Leica Confocal Software. | ||
# Choose “Personal” and start the initialization process. | # Choose “Personal” and start the initialization process. | ||
| − | === | + | === Basic Acquisition Setup === |
[[image:mpi03_swtoolbar.jpg|200x100px]] [[image:mpi03_beampath.jpg|200x100px]] [[image:mpi03_controlpanel.jpg|200x100px]] | [[image:mpi03_swtoolbar.jpg|200x100px]] [[image:mpi03_beampath.jpg|200x100px]] [[image:mpi03_controlpanel.jpg|200x100px]] | ||
# In the Aquire toolbar, select beam to open the Beam Path Setting window. | # In the Aquire toolbar, select beam to open the Beam Path Setting window. | ||
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# Use the control panel to adjust for image gain and offset | # Use the control panel to adjust for image gain and offset | ||
| − | === | + | === Manual Setup - Hints === |
[[image:mpi03_stand.jpg|200x100px]] | [[image:mpi03_stand.jpg|200x100px]] | ||
Revision as of 17:19, 17 June 2009
Contents |
How to start the Leica TCS SP2
Control Panel
- Switch buttons from right to left (PC-Scanner-Laser)
- Before switching on the argon laser, check that the power Level is set to MIN. Once the laser is switched on you, can turn the power up (labeled mark).
- Argon laser for laser lines: 458, 477, 488, 514. To switch on first turn the key to START, it then jumps automatically back to the ON position.
- Switch on 543nm and 633nm laser line if needed.
Software Initiation
- Make sure that the upper stage limit is set before starting the Leica Confocal Software.
- Choose “Personal” and start the initialization process.
Basic Acquisition Setup
- In the Aquire toolbar, select beam to open the Beam Path Setting window.
- There you can set up all the aquisition parameters according to your sample and needs.
- Use the control panel to adjust for image gain and offset
Manual Setup - Hints
NOTE : This is a rather manual set up! YOU HAVE TO ...
- switch between scanning and visible mode ...
- change filter wheels (GFP, RFP filter for observation by eye, empty position for scanning) ...
- change objectives ...
... BY HAND!!!
