MPI-CL1 - Leica TCS SP2 - startup & shutdown

How to start the Leica TCS SP2

Hardware

1. Switch buttons from right to left (PC-Scanner-Laser)
2. Before switching on the argon laser, check that the power Level is set to MIN. Once the laser is switched on you, can turn the power up (labeled mark).
3. Argon laser for laser lines: 458, 477, 488, 514. To switch on first turn the key to START, it then jumps automatically back to the ON position.
4. Switch on 543nm and 633nm laser line if needed.

Software Initiation

1. Make sure that the upper stage limit is set before starting the Leica Confocal Software.
2. Choose “Personal” and start the initialization process.

Basic Acquisition Setup

1. In the Aquire toolbar, select beam to open the Beam Path Setting window.
2. There you can set up all the aquisition parameters according to your sample and needs.
3. Use the control panel to adjust for image gain and offset

Manual Setup - Hints

NOTE : This is a rather manual set up! YOU HAVE TO ...

• switch between scanning and visible mode ...
• change filter wheels (GFP, RFP filter for observation by eye, empty position for scanning) ...
• change objectives ...

... BY HAND!!!