MPI-CO1 - Olympus FV1000 - startup & shutdown
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== How to start the Olympus FV1000 == | == How to start the Olympus FV1000 == | ||
− | === | + | === Hardware === |
[[image:mpi09_561.jpg|200x100px]] | [[image:mpi09_561.jpg|200x100px]] | ||
[[image:mpi09_argon-633.jpg|200x100px]] | [[image:mpi09_argon-633.jpg|200x100px]] | ||
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#* LD405 ... 405nm imaging laser | #* LD405 ... 405nm imaging laser | ||
#* 405nm cutter ... bleaching/ ablation laser | #* 405nm cutter ... bleaching/ ablation laser | ||
− | === | + | === Software Initiation=== |
− | [[image:mpi09_sw-icon.jpg|200x100px]] [[image:mpi09_sw-login | + | [[image:mpi09_sw-icon.jpg|200x100px]] [[image:mpi09_sw-login.jpg|200x100px]] |
# Start software and log in as lmf user. | # Start software and log in as lmf user. | ||
+ | |||
+ | === Basic Acquisition Setup === | ||
+ | [[image:mpi09_sw-dyelist.jpg|200x100px]] [[image:mpi09_sw-acquisition.jpg|200x100px]] [[image:mpi09_sw-explorer.jpg|200x100px]] | ||
# If applicable, use the Olympus Dye list to help you set up the beam path settings. | # If applicable, use the Olympus Dye list to help you set up the beam path settings. | ||
# Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background. | # Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background. | ||
# You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters” | # You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters” | ||
− | === | + | === Basic Troubleshooting === |
[[image:mpi09_trouble-shutter.jpg|200x100px]] [[image:mpi09_trouble-sim.jpg|200x100px]] | [[image:mpi09_trouble-shutter.jpg|200x100px]] [[image:mpi09_trouble-sim.jpg|200x100px]] | ||
* PROBLEM: No light at the object plane! | * PROBLEM: No light at the object plane! |
Revision as of 17:28, 17 June 2009
Contents |
How to start the Olympus FV1000
Hardware
- Switch on lasers you need:
- 633nm laser: turn key
- Argon laser: switch button, then turn the key
- 561nm laser: press the green button
- Switch on the motorised stage
- Switch on the rest of the system using the power manager below the monitors. Think about what components you really need (otherwise leave them switched off):
- LD405 ... 405nm imaging laser
- 405nm cutter ... bleaching/ ablation laser
Software Initiation
- Start software and log in as lmf user.
Basic Acquisition Setup
- If applicable, use the Olympus Dye list to help you set up the beam path settings.
- Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background.
- You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters”
Basic Troubleshooting
- PROBLEM: No light at the object plane!
- CHECK: excitation shutter of turret open??
- PROBLEM: No 405nm imaging laser at the object plane!
- CHECK: SIM function disabled??