MPI-CO1 - Olympus FV1000 - startup & shutdown
From BioDIP
Contents |
How to initiate the Olympus FV1000
hardware
- Switch on lasers you need:
- 633nm laser: turn key
- Argon laser: switch button, then turn the key
- 561nm laser: press the green button
- Switch on the motorised stage
- Switch on the rest of the system using the power manager below the monitors. Think about what components you really need (otherwise leave them switched off):
- LD405 ... 405nm imaging laser
- 405nm cutter ... bleaching/ ablation laser
software
- Start software and log in as lmf user.
- If applicable, use the Olympus Dye list to help you set up the beam path settings.
- Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background.
- You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters”
basic troubleshooting
- PROBLEM: No light at the object plane!
- CHECK: excitation shutter of turret open??
- PROBLEM: No 405nm imaging laser at the object plane!
- CHECK: SIM function disabled??
software initiation
- Make sure that the upper stage limit is set before starting the Leica Confocal Software.
- Choose “Personal” and start the initialization process.
