MZ1 - Zeiss 2photon upright
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{{Equipment | {{Equipment | ||
− | |system-number=MPI-MZ1 | + | |system-number=MPI- MZ1 |
|system-name=Zeiss 2-photon upright | |system-name=Zeiss 2-photon upright | ||
|type=Microscope | |type=Microscope | ||
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|Facility-logo=MPI-CBG-Lmf-logo-big.png | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
− | |description=Zeiss LSM 780 NLO | + | |description=Zeiss LSM 780 NLO<br>Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.<br>Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition |
− | + | ||
− | + | ||
|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | ||
|image=MZ1 - Zeiss 2photon upright.jpg | |image=MZ1 - Zeiss 2photon upright.jpg | ||
− | |stand-name=Zeiss - Axio | + | |stand-name=Zeiss - Axio Examiner.Z1 |
|stand=Upright | |stand=Upright | ||
|microscope=motorized XY stage, motorized Z-drive, | |microscope=motorized XY stage, motorized Z-drive, | ||
|obj1=Zeiss EC Plan-Neofluar 10x 0.3 | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 | ||
− | |obj2=Zeiss | + | |obj2=Zeiss Plan-Apochromat 10x 0.45 |
− | |obj3=Zeiss Plan-Apochromat | + | |obj3=Zeiss Clr Plan-Apochromat 10x 0.5 Scale |
|obj4=Zeiss N-Achroplan 20x 0.5 W | |obj4=Zeiss N-Achroplan 20x 0.5 W | ||
|obj5=Zeiss Plan-Apochromat 20x 0.8 | |obj5=Zeiss Plan-Apochromat 20x 0.8 | ||
− | |obj6=Zeiss Plan-Apochromat | + | |obj6=Zeiss W Plan-Apochromat 20x 1.0 DIC |
− | |obj7=Zeiss Plan- | + | |obj7=Zeiss Clr Plan-Neofluar 20x 1.0 Korr Clarity |
− | |obj8=Zeiss | + | |obj8=Zeiss LD SC Plan-Apochromat 20x 1.0 Korr Scale |
|obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC | |obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC | ||
− | |ill1=Fluorescence (Metal Halide, | + | |obj10=Zeiss C-Apochromat 40x 1.2 W |
+ | |obj11=Zeiss Plan-Apochromat 40x 1.3 Oil DIC | ||
+ | |obj12=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC | ||
+ | |obj13=Zeiss Plan-Apochromat 63x 1.4 Oil DIC | ||
+ | |ill1=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill2=Transmitted Light (Halogen) | |ill2=Transmitted Light (Halogen) | ||
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
− | |ill4=Laser Argon Multiline 458 | + | |ill4=Laser Argon Multiline 458, 488, 514 nm |
|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser HeNe 594 nm | |ill6=Laser HeNe 594 nm | ||
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|ill8=Laser Multiphoton Chameleon 700-1064 nm | |ill8=Laser Multiphoton Chameleon 700-1064 nm | ||
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | |detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | ||
− | '''2 photon detection:''' | + | '''2 photon detection:''' 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction |
|reflectors=Zeiss set 49: DAPI<br> | |reflectors=Zeiss set 49: DAPI<br> | ||
Zeiss set 38 Endow: GFP<br> | Zeiss set 38 Endow: GFP<br> |
Latest revision as of 19:23, 3 February 2016
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https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details