MZ1 - Zeiss 2photon upright
From BioDIP
(Difference between revisions)
(4 intermediate revisions by one user not shown) | |||
Line 9: | Line 9: | ||
|Facility-logo=MPI-CBG-Lmf-logo-big.png | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
− | |description=Zeiss LSM 780 NLO | + | |description=Zeiss LSM 780 NLO<br>Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.<br>Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition |
− | + | ||
− | + | ||
|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | ||
|image=MZ1 - Zeiss 2photon upright.jpg | |image=MZ1 - Zeiss 2photon upright.jpg | ||
Line 27: | Line 25: | ||
|obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC | |obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC | ||
|obj10=Zeiss C-Apochromat 40x 1.2 W | |obj10=Zeiss C-Apochromat 40x 1.2 W | ||
+ | |obj11=Zeiss Plan-Apochromat 40x 1.3 Oil DIC | ||
+ | |obj12=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC | ||
+ | |obj13=Zeiss Plan-Apochromat 63x 1.4 Oil DIC | ||
|ill1=Fluorescence (Metal Halide, HXP, 120W) | |ill1=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill2=Transmitted Light (Halogen) | |ill2=Transmitted Light (Halogen) | ||
Line 36: | Line 37: | ||
|ill8=Laser Multiphoton Chameleon 700-1064 nm | |ill8=Laser Multiphoton Chameleon 700-1064 nm | ||
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | |detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | ||
− | '''2 photon detection:''' | + | '''2 photon detection:''' 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction |
|reflectors=Zeiss set 49: DAPI<br> | |reflectors=Zeiss set 49: DAPI<br> | ||
Zeiss set 38 Endow: GFP<br> | Zeiss set 38 Endow: GFP<br> | ||
Line 46: | Line 47: | ||
|link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedC1CZjIxa05nTnFOM2Ixb0RvdGltaEE&output=html | |link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedC1CZjIxa05nTnFOM2Ixb0RvdGltaEE&output=html | ||
|inv=AIM System 2501000445 10.1.145.41 (IPv4) | |inv=AIM System 2501000445 10.1.145.41 (IPv4) | ||
− | |||
− | |||
− | |||
}} | }} |
Latest revision as of 19:23, 3 February 2016
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details