MZ1 - Zeiss 2photon upright
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{{Equipment | {{Equipment | ||
| − | |system-number=MPI-MZ1 | + | |system-number=MPI- MZ1 |
|system-name=Zeiss 2-photon upright | |system-name=Zeiss 2-photon upright | ||
|type=Microscope | |type=Microscope | ||
| Line 7: | Line 7: | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=038C | |room=038C | ||
| − | |description=Zeiss LSM 780 NLO | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| − | + | |Institute-logo=Logo MPI-CBG.jpg | |
| − | + | |description=Zeiss LSM 780 NLO<br>Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.<br>Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition | |
|applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | ||
| − | |image=MZ1-Zeiss 2photon | + | |image=MZ1 - Zeiss 2photon upright.jpg |
| − | |stand-name=Zeiss - Axio | + | |stand-name=Zeiss - Axio Examiner.Z1 |
|stand=Upright | |stand=Upright | ||
|microscope=motorized XY stage, motorized Z-drive, | |microscope=motorized XY stage, motorized Z-drive, | ||
|obj1=Zeiss EC Plan-Neofluar 10x 0.3 | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 | ||
| − | |obj2=Zeiss | + | |obj2=Zeiss Plan-Apochromat 10x 0.45 |
| − | |obj3=Zeiss | + | |obj3=Zeiss Clr Plan-Apochromat 10x 0.5 Scale |
| − | |obj4=Zeiss | + | |obj4=Zeiss N-Achroplan 20x 0.5 W |
|obj5=Zeiss Plan-Apochromat 20x 0.8 | |obj5=Zeiss Plan-Apochromat 20x 0.8 | ||
| − | |obj6=Zeiss Plan-Apochromat | + | |obj6=Zeiss W Plan-Apochromat 20x 1.0 DIC |
| − | |obj7=Zeiss Plan-Apochromat | + | |obj7=Zeiss Clr Plan-Neofluar 20x 1.0 Korr Clarity |
| − | | | + | |obj8=Zeiss LD SC Plan-Apochromat 20x 1.0 Korr Scale |
| − | |ill1=Fluorescence (Metal Halide, | + | |obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC |
| + | |obj10=Zeiss C-Apochromat 40x 1.2 W | ||
| + | |obj11=Zeiss Plan-Apochromat 40x 1.3 Oil DIC | ||
| + | |obj12=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC | ||
| + | |obj13=Zeiss Plan-Apochromat 63x 1.4 Oil DIC | ||
| + | |ill1=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill2=Transmitted Light (Halogen) | |ill2=Transmitted Light (Halogen) | ||
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
| − | |ill4=Laser Argon Multiline 458 | + | |ill4=Laser Argon Multiline 458, 488, 514 nm |
|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser HeNe 594 nm | |ill6=Laser HeNe 594 nm | ||
| Line 32: | Line 37: | ||
|ill8=Laser Multiphoton Chameleon 700-1064 nm | |ill8=Laser Multiphoton Chameleon 700-1064 nm | ||
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | |detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | ||
| − | '''2 photon detection:''' | + | '''2 photon detection:''' 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction |
| − | |reflectors= | + | |reflectors=Zeiss set 49: DAPI<br> |
| + | Zeiss set 38 Endow: GFP<br> | ||
| + | Zeiss set 45: HQ Texas Red | ||
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
| − | |software=Zen | + | |software=Zen 2012 black |
|incubation=Not available | |incubation=Not available | ||
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-MZ1_-_Zeiss_2photon_upright_-_startup_%26_shutdown | |link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-MZ1_-_Zeiss_2photon_upright_-_startup_%26_shutdown | ||
| − | |link1=https:// | + | |link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedC1CZjIxa05nTnFOM2Ixb0RvdGltaEE&output=html |
|inv=AIM System 2501000445 10.1.145.41 (IPv4) | |inv=AIM System 2501000445 10.1.145.41 (IPv4) | ||
}} | }} | ||
Latest revision as of 19:23, 3 February 2016
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