MZ1 - Zeiss 2photon upright

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m (adjusting system id to the form of LZ2)
(ordered objectives after magnification and NA)
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|microscope=motorized XY stage, motorized Z-drive,
 
|microscope=motorized XY stage, motorized Z-drive,
 
|obj1=Zeiss EC Plan-Neofluar 10x 0.3
 
|obj1=Zeiss EC Plan-Neofluar 10x 0.3
|obj2=Zeiss C-Apochromat 40x 1.2 W
+
|obj2=Zeiss Plan-Apochromat 10x 0.45
|obj3=Zeiss Plan-Apochromat 40x 1.0 W DIC
+
|obj3=Zeiss N-Achroplan 20x 0.5 W
|obj4=Zeiss N-Achroplan 20x 0.5 W
+
|obj4=Zeiss Plan-Apochromat 20x 0.8
|obj5=Zeiss Plan-Apochromat 20x 0.8
+
|obj5=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
|obj6=Zeiss Plan-Apochromat 10x 0.45
+
|obj6=Zeiss C-Apochromat 40x 1.2 W
|obj7=Zeiss Plan-Apochromat 63x 1.4 Oil DIC
+
|obj7=Zeiss Plan-Apochromat 40x 1.0 W DIC
 
|obj8=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
 
|obj8=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
|obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
+
|obj9=Zeiss Plan-Apochromat 63x 1.4 Oil DIC
 +
 
 
|ill1=Fluorescence (Metal Halide, PRIOR, 200W)
 
|ill1=Fluorescence (Metal Halide, PRIOR, 200W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)

Revision as of 18:55, 3 February 2016

  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
  • Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
  • Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS.
MZ1 - Zeiss 2photon upright.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038C)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized Z-drive,
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss Plan-Apochromat 10x 0.45
Zeiss N-Achroplan 20x 0.5 W
Zeiss Plan-Apochromat 20x 0.8
Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
Zeiss C-Apochromat 40x 1.2 W
Zeiss Plan-Apochromat 40x 1.0 W DIC
Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
Zeiss Plan-Apochromat 63x 1.4 Oil DIC
illumination
Fluorescence (Metal Halide, PRIOR, 200W)
Transmitted Light (Halogen)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Laser Multiphoton Chameleon 700-1064 nm
detection Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting

2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP

reflectors Zeiss set 49: DAPI

Zeiss set 38 Endow: GFP
Zeiss set 45: HQ Texas Red

features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software Zen 2012 black
incubation Not available
links
  • [ beam path]
  • [ advanced system check]
inv.nr. AIM System 2501000445 10.1.145.41 (IPv4)



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